Effect of the Cry1, Cry2 and Vip3 protein combinations on the control ofAnticarsia gemmatalis(Erebidae) andChrysodeixis includens(Noctuidae) Lepidoptera

被引：3

作者：

Figueiredo, Camila Soares ^{[1
]}

Zara, Fernando Jose ^{[2
]}

Desiderio, Janete Apparecida ^{[1
]}

机构：

[1] UNESP Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Biol Aplicada Agr, Jaboticabal, SP, Brazil

[2] UNESP, IEAMAR, Aquaculture Ctr CAUNESP, Sao Vicente, SP, Brazil

来源：

INTERNATIONAL JOURNAL OF PEST MANAGEMENT | 2022年 / 68卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

Synergism; antagonism; heterologous competition; resistance management; soybean looper; velvetbean caterpillar; SPODOPTERA-FRUGIPERDA MIDGUT; BACILLUS-THURINGIENSIS CRY1; ANTICARSIA-GEMMATALIS; HELICOVERPA-ARMIGERA; BINDING-SITES; LARVAL MIDGUT; NOCTUIDAE; TOXIN; RESISTANCE; SUSCEPTIBILITY;

D O I：

10.1080/09670874.2020.1817617

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

The transgenic soybean expressing thecry1Acinsecticidal gene of theBacillus thuringiensisbacterium has been widely adopted in agriculture. This bacterium acts as an efficient control agent of pest insects due to the expression of different proteins with entomopathogenic activity such as Cry and Vip. This study investigates the toxicity and mode of interaction between the Cry1Ab, Cry1Ac, Cry2Aa, Cry2Ab and Vip3Aa proteins againstChrysodeixis includensandAnticarsia gemmatalislarvae. The combination of the Cry1Ac, Cry2Aa and Vip3Aa toxins had a synergistic effect forC. includens, however, only Vip3Aa and Cry2Aa exhibited synergistic interaction againstA. gemmatalis. Cry1Ab and Cry1Ac proteins have been shown to share receptors with the two species tested, however, Cry1Ac does not share binding sites with Cry2Aa, Cry2Ab, and Vip3Aa in both species. Besides helping the resistance management toB. thuringiensisproteins, the combination of Cry and Vip proteins can increase toxicity through synergistic action.

引用

页码：175 / 183

页数：9

共 41 条

[1] Study of the Bacillus thuringiensis Vip3Aa16 histopathological effects and determination of its putative binding proteins in the midgut of Spodoptera littoralis [J].

Abdelkefi-Mesrati, Lobna ;

Boukedi, Hanen ;

Dammak-Karray, Mariam ;

Sellami-Boudawara, Tahya ;

Jaoua, Samir ;

Tounsi, Slim .

JOURNAL OF INVERTEBRATE PATHOLOGY, 2011, 106 (02) :250-254

[2]

[Anonymous], 2016, TRENDS BIOTECHNOL, DOI DOI 10.1016/j.tibtech.2015.12.011

[3]

Bel Y, 2017, APPL ENVIRON MICROB, V83, DOI [10.1128/AEM.00326-17, 10.1128/aem.00326-17]

[4] Histopathological effects and determination of the putative receptor of Bacillus thuringiensis Cry1Da toxin in Spodoptera littoralis midgut [J].

BenFarhat-Touzri, Dalel ;

Saadaoui, Marwa ;

Abdelkefi-Mesrati, Lobna ;

Saadaoui, Imen ;

Azzouz, Hichem ;

Tounsi, Slim .

JOURNAL OF INVERTEBRATE PATHOLOGY, 2013, 112 (02) :142-145

[5] Bacillus thuringiensis Cry1Ia10 and Vip3Aa protein interactions and their toxicity in Spodoptera spp. (Lepidoptera) [J].

Bergamasco, V. B. ;

Mendes, D. R. P. ;

Fernandes, O. A. ;

Desiderio, J. A. ;

Lemos, M. V. F. .

JOURNAL OF INVERTEBRATE PATHOLOGY, 2013, 112 (02) :152-158

[6] Baseline Susceptibility and Monitoring of Brazilian Populations of Spodoptera frugiperda (Lepidoptera: Noctuidae) and Diatraea saccharalis (Lepidoptera: Crambidae) to Vip3Aa20 Insecticidal Protein [J].

Bernardi, Oderlei ;

Amado, Douglas ;

Sousa, Renan S. ;

Segatti, Fabiana ;

Fatoretto, Julio ;

Burd, Anthony D. ;

Omoto, Celso .

JOURNAL OF ECONOMIC ENTOMOLOGY, 2014, 107 (02) :781-790

[7] Assessment of the high-dose concept and level of control provided by MON 87701 x MON 89788 soybean against Anticarsia gemmatalis and Pseudoplusia includens (Lepidoptera: Noctuidae) in Brazil [J].

Bernardi, Oderlei ;

Malvestiti, Glaucia S. ;

Dourado, Patrick M. ;

Oliveira, Wladecir S. ;

Martinelli, Samuel ;

Berger, Geraldo U. ;

Head, Graham P. ;

Omoto, Celso .

PEST MANAGEMENT SCIENCE, 2012, 68 (07) :1083-1091

[8]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[9] Interaction of Cry1 and Vip3A proteins of Bacillus thuringiensis for the control of lepidopteran insect pests [J].

Brunini Crialesi-Legori, Paula Cristina ;

Davolos, Camila Chiaradia ;

Nunes Lemes, Ana Rita ;

Marucci, Suzana Cristina ;

Franco Lemos, Manoel Victor ;

Fernandes, Odair Aparecido ;

Desiderio, Janete Apparecida .

PESQUISA AGROPECUARIA BRASILEIRA, 2014, 49 (02) :79-87

[10] Dominant Negative Phenotype of Bacillus thuringiensis Cry1Ab, Cry11Aa and Cry4Ba Mutants Suggest Hetero-Oligomer Formation among Different Cry Toxins [J].

Carmona, Daniela ;

Rodriguez-Almazan, Claudia ;

Munoz-Garay, Carlos ;

Portugal, Leivi ;

Perez, Claudia ;

de Maagd, Ruud A. ;

Bakker, Petra ;

Soberon, Mario ;

Bravo, Alejandra .

PLOS ONE, 2011, 6 (05)

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