Inhibitory effects of trolox-encapsulated chitosan nanoparticles on tert-butylhydroperoxide induced RAW264.7 apoptosis

被引：50

作者：

Han, Lu ^{[1
]}

Du, Li-Bo ^{[1
]}

Kumar, Anil ^{[2
]}

Jia, Hong-Ying ^{[1
]}

Liang, Xing-Jie ^{[2
]}

Tian, Qiu ^{[1
]}

Nie, Guang-Jun ^{[2
]}

Liu, Yang ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100190, Peoples R China

[2] Chinese Acad Sci, Natl Ctr Nanosci & Technol, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China

来源：

BIOMATERIALS | 2012年 / 33卷 / 33期

基金：

中国国家自然科学基金;

关键词：

Trolox; Chitosan nanoparticles; Anti-Oxidation activities; Anti-Apoptosis effects; POLYMERIC MICELLES; IN-VITRO; ANTIOXIDANT; DELIVERY; BIOAVAILABILITY; CHITIN; DEATH;

D O I：

10.1016/j.biomaterials.2012.07.034

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A nanocarrier, namely, hydroxylethyl-chitosan nanoparticles was developed in this research for delivering antioxidants with 6-hydroxy-2, 5, 7, 8-tetra-methylchromane-2-carboxylic acid (trolox) as a model antioxidant. The trolox-encapsulated chitosan nanoparticles (trolox-CS NPs) were prepared by modifying chitosan with epoxyethane, which self-assembled into NPs and entrapped trolox, and then characterized by their size, size distribution, morphology and in vitro trolox release profile. Intracellular trafficking of CS NPs was observed. The anti-oxidant effect and potential mechanism of trolox-CS NPs were subsequently investigated in RAW264.7 cells. The effects of trolox-CS NPs on RAW264.7 cells damaged by tert-butylhydroperoxide (t-BHP) were determined by MTT assay for cell viability, MDA assay for membrane lipid peroxidation, JC-1 probe and Annexin V-FITC/PI double staining for mitochondria membrane potential (MMP) and RAW264.7 apoptosis, respectively. The trolox-CS NPs significantly improved cell viability and reduced MDA content compared with those of cells treated with free trolox. The trolox-CS NPs treatment inhibited MMP collapse and RAW264.7 apoptosis more obviously than free trolox. Molecular basis of apoptosis studied by western blotting revealed that trolox-CS NPs may block mitochondria-mediated apoptosis pathway through up-regulation of Bcl-2 and down-regulation of Bax and inhibiting the activation of pro-caspase 3, PARP and Bid. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：8517 / 8528

页数：12

共 45 条

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