Oligonucleotide adsorption to gold nanoparticles: A surface-enhanced raman spectroscopy study of intrinsically bent DNA

被引：175

作者：

Gearheart, LA ^{[1
]}

Ploehn, HJ ^{[1
]}

Murphy, CJ ^{[1
]}

机构：

[1] Univ S Carolina, Dept Chem & Biochem, Columbia, SC 29208 USA

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2001年 / 105卷 / 50期

关键词：

D O I：

10.1021/jp0106606

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We have examined the adsorption of short 16 base pair DNA to 14 nm protein-sized gold particles using surface-enhanced Raman spectroscopy and electronic absorption spectroscopy. In the presence of KCI, we find greater Raman enhancement of adenine and guanine ring breathing vibrations (per adenine and guanine present in each sequence) for crystallographically "kinked" oligonucleotide than for the DNA-particle interactions for "bent" A-tract and "straight" oligonucleotides. The calculated binding constants are within the range of nonspecific protein-DNA interactions and are nearly the same for each sequence. The difference in the surface plasmon frequency of the gold particles in the presence of either straight, bent, or kinked DNA is attributed to differences in the DNA-mediated aggregation of the nanoparticles. Specifically, the displacement of the gold's stabilizing citrate ions seems to vary with DNA sequence, and this controls the residual interparticle electrostatic interaction and average interparticle separation in the aggregate.

引用

页码：12609 / 12615

页数：7

共 92 条

[1] Organization of 'nanocrystal molecules' using DNA [J].

Alivisatos, AP ;

Johnsson, KP ;

Peng, XG ;

Wilson, TE ;

Loweth, CJ ;

Bruchez, MP ;

Schultz, PG .

NATURE, 1996, 382 (6592) :609-611

[2]

ANDERSON CF, 1990, ANNU REV BIOPHYS BIO, V19, P423

[3] Three-dimensional structure of the Stat3β homodimer bound to DNA [J].

Becker, S ;

Groner, B ;

Müller, CW .

NATURE, 1998, 394 (6689) :145-151

[4] Measurement of local DNA reorganization on the picosecond and nanosecond time scales [J].

Brauns, EB ;

Madaras, ML ;

Coleman, RS ;

Murphy, CJ ;

Berg, MA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (50) :11644-11649

[5] Local dynamics in DNA by temperature-dependent Stokes shifts of an intercalated dye [J].

Brauns, EB ;

Murphy, CJ ;

Berg, MA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (10) :2449-2456

[6] PHYSIOLOGICAL CONCENTRATION OF MAGNESIUM-IONS INDUCES A STRONG MACROSCOPIC CURVATURE IN GGGCCC-CONTAINING DNA [J].

BRUKNER, I ;

SUSIC, S ;

DLAKIC, M ;

SAVIC, A ;

PONGOR, S .

JOURNAL OF MOLECULAR BIOLOGY, 1994, 236 (01) :26-32

[7] EVIDENCE FOR OPPOSITE GROOVE-DIRECTED CURVATURE OF GGGCCC AND AAAAA SEQUENCE ELEMENTS (VOL 21, PG 1025, 1993) [J].

BRUKNER, I ;

DLAKIC, M ;

SAVIC, A ;

SUSIC, S ;

PONGER, S ;

SUCK, D .

NUCLEIC ACIDS RESEARCH, 1993, 21 (05) :1332-1332

[8] EVIDENCE FOR OPPOSITE GROOVE-DIRECTED CURVATURE OF GGGCCC AND AAAAA SEQUENCE ELEMENTS [J].

BRUKNER, I ;

DLAKIC, M ;

SAVIC, A ;

SUSIC, S ;

PONGOR, S ;

SUCK, D .

NUCLEIC ACIDS RESEARCH, 1993, 21 (04) :1025-1029

[9] Interaction of DNA base pairs with various metal cations (Mg2+, Ca2+, Sr2+, Ba2+, Cu+, Ag+, Au+, Zn2+, Cd2+, and Hg2+): Nonempirical ab initio calculations on structures, energies, and nonadditivity of the interaction [J].

Burda, JV ;

Sponer, J ;

Leszczynski, J ;

Hobza, P .

JOURNAL OF PHYSICAL CHEMISTRY B, 1997, 101 (46) :9670-9677

[10] Ab Initio study of the interaction of guanine and adenine with various mono- and bivalent metal cations (Li+, Na+, K+, Rb+, Cs+; Cu+, Ag+, Au+; Mg2+, Ca2+, Sr2+, Ba2+; Zn2+, Cd2+, and Hg2+) [J].

Burda, JV ;

Sponer, J ;

Hobza, P .

JOURNAL OF PHYSICAL CHEMISTRY, 1996, 100 (17) :7250-7255

← 1 2 3 4 5 6 7 8 9 10 →