Effect of eluent pH on the HPLC-UV analysis of hyperforin from St. John's Wort (Hypericum perforatum L.)

被引：12
作者：
Fourneron, JD [1 ]
Naït-Si, Y [1 ]
机构：
[1] Fac Pharm Marseille, Lab Galen & Ind Pharm, F-13385 Marseille, France
来源：
PHYTOCHEMICAL ANALYSIS | 2006年 / 17卷 / 02期
关键词：
HPLC-PAD analysis; UV spectroscopy; hyperforin; St. John's wort;
D O I：
10.1002/pca.888
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The effect of the pH of the mobile phase in HPLC analysis of hyperforin was investigated. Working with an extract of St. John's Wort (Hypericum perforatum. L.) that is rich in hyperforin, significant differences were observed in conventional chromatograms depending on whether the mobile phase was acidic or alkaline. Chromatogram changes were paralleled by changes in the UV spectrum of the hyperforin peak. The structural changes In hyperforin occur in the chromatographic column itself, as has been confirmed by UV spectroscopy performed on a sample of purified hyperforin, which showed that the UV spectrum is indeed dependent on the pH of its environment. Copyright (C) 2005 John Wiley & Sons, Ltd.
引用
收藏
页码：71 / 77
页数：7
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