Immunosensor Based on Immobilization of Antigenic Peptide NS5A-1 from HCV and Silk Fibroin in Nanostructured Films

被引：26

作者：

Moraes, Marli L. ^{[1
]}

Lima, Lais R. ^{[1
]}

Silva, Robson R. ^{[1
]}

Cavicchioli, Mauricio ^{[1
]}

Ribeiro, Sidney J. L. ^{[1
]}

机构：

[1] Sao Paulo State Univ, UNESP, Inst Chem, BR-14801970 Araraquara, SP, Brazil

来源：

LANGMUIR | 2013年 / 29卷 / 11期

基金：

巴西圣保罗研究基金会;

关键词：

BIOMATERIAL COATINGS; PHYSICAL-PROPERTIES; CONTROLLED-RELEASE; CHITOSAN; LAYER; ADSORPTION; PROTEIN; BIOSENSORS; OXIDASE;

D O I：

10.1021/la304404v

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The peptide NS5A-1 (PPLLESWICDP-DYVPPWHG), derived from hepatitis C virus (HCV) NS5A protein, was immobilized into layer-by-layer (LbL) silk fibroin (SF) films. Deposition was monitored by UV-vis absorption measurements at each bilayer deposited. The interaction SF/peptide film induced secondary structure in NS5A-1 as indicated by fluorescence and circular dichroism (CD) measurements. Voltammetric sensor (SF/NS5A-1) properties were observed when the composite film was tested in the presence of anti-HCV. The peptide silk fibroin interaction studied here showed new architectures for immunosensors based on antigenic peptides and SF as a suitable immobilization matrix.

引用

页码：3829 / 3834

页数：6

共 39 条

[1] Assembling alternate dye-polyion molecular films by electrostatic layer-by-layer adsorption [J].

Ariga, K ;

Lvov, Y ;

Kunitake, T .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (09) :2224-2231

[2] Biomaterials and Biofunctionality in Layered Macromolecular Assemblies [J].

Ariga, Katsuhiko ;

Hill, Jonathan P. ;

Ji, Qingmin .

MACROMOLECULAR BIOSCIENCE, 2008, 8 (11) :981-990

[3] Peptides and liposomes: From biophysical to immunogenic studies [J].

Busquets, MA ;

Alsina, MA ;

Haro, I .

CURRENT DRUG TARGETS, 2003, 4 (08) :633-642

[4] Surface engineering of titanium thin films with silk fibroin via layer-by-layer technique and its effects on osteoblast growth behavior [J].

Cai, Kaiyong ;

Hu, Yan ;

Jandt, Klaus D. .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2007, 82A (04) :927-935

[5] Fuzzy nanoassemblies: Toward layered polymeric multicomposites [J].

Decher, G .

SCIENCE, 1997, 277 (5330) :1232-1237

[6] ANTIGENIC PEPTIDES [J].

DYSON, HJ ;

WRIGHT, PE .

FASEB JOURNAL, 1995, 9 (01) :37-42

[7] Physical properties and structure of poly(ethylene glycol)-silk fibroin conjugate films [J].

Gotoh, Y ;

Tsukada, M ;

Baba, T ;

Minoura, N .

POLYMER, 1997, 38 (02) :487-490

[8] Mechanical properties of robust ultrathin silk fibroin films [J].

Jiang, Chaoyang ;

Wang, Xianyan ;

Gunawidjaja, Ray ;

Lin, Yen-Hsi ;

Gupta, Maneesh K. ;

Kaplan, David L. ;

Naik, Rajesh R. ;

Tsukruk, Vladimir V. .

ADVANCED FUNCTIONAL MATERIALS, 2007, 17 (13) :2229-2237

[9] The effect of the chitosan membrane properties on the enzyme adsorption and performance for the construction of horseradish peroxidase biosensors [J].

Jin, Xin ;

Xi, Fengna ;

Lv, Deshui ;

Wu, Qi ;

Lin, Xianfu .

CARBOHYDRATE POLYMERS, 2011, 85 (04) :786-791

[10] Application of chitin- and chitosan-based materials for enzyme immobilizations: a review [J].

Krajewska, B .

ENZYME AND MICROBIAL TECHNOLOGY, 2004, 35 (2-3) :126-139

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