Purification and characterization of polyphenol oxidase from fresh ginseng

被引：14

作者：

Kim, Jae-Joon ^{[1
]}

Kim, Woo-Yeon ^{[1
]}

机构：

[1] Chung Ang Univ, Dept Biotechnol, Anseong 456756, South Korea

来源：

JOURNAL OF GINSENG RESEARCH | 2013年 / 37卷 / 01期

关键词：

Panax ginseng; Polyphenol oxidase; Purification; Catechol; Carboxymethyl-Sepharose;

D O I：

10.5142/jgr.2013.37.117

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Polyphenol oxidase (PPO) was purified from fresh ginseng roots using acetone precipitation, carboxymethyl (CM)-Sepharose chromatography, and phenyl-Sepharose chromatography. Two isoenzymes (PPO 1 and PPO 2) were separated using an ion-exchange column with CM-Sepharose. PPO 1 was purified up to 13.2-fold with a 22.6% yield. PPO 2 bound to CM-Sepharose, eluted with NaCl, and was purified up to 22.5-fold with a 17.4% yield. PPO 2 was further chromatographed on phenyl-Sepharose. The molecular weight of the purified PPO 2 from fresh ginseng was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and was about 40 kDa. The optimum temperature and pH were 20 degrees C and 7.0, respectively, using catechol as a substrate. Pyrogallol showed the highest substrate specificity. The effect of a PPO inhibitor showed that its activity increased slightly in the presence of a low concentration of citric acid. High concentrations of acidic compounds and sulfite agents significantly inhibited purified ginseng PPO 2.

引用

页码：117 / 123

页数：7

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