In vitro antiplasmodial activity of selected Luo and Kuria medicinal plants

被引：32

作者：

Owuor, B. O. ^{[1
,2
]}

Ochanda, J. O. ^{[2
]}

Kokwaro, J. O. ^{[3
]}

Cheruiyot, A. C. ^{[4
]}

Yeda, R. A. ^{[4
]}

Okudo, C. A. ^{[4
]}

Akala, H. M. ^{[4
]}

机构：

[1] Catholic Univ Eastern Africa, Dept Biol, Nairobi, Kenya

[2] Univ Nairobi, Ctr Biotechnol & Bioinformat, Nairobi, Kenya

[3] Univ Nairobi, Sch Biol Sci, Nairobi, Kenya

[4] Kenya Med Res Inst KEMRI, Dept Emerging Infect Dis DEID Program, USAMRU K, Walter Reed Project, Kisian Kisumu, Kenya

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2012年 / 144卷 / 03期

关键词：

Medicinal plants; Antiplasmodial activity; Traditional medicine Africa; PLASMODIUM-FALCIPARUM; MALARIA; QUINOLINES; KENYA; ASSAY;

D O I：

10.1016/j.jep.2012.09.045

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Ethnopharmacological relevance: Drug resistance in malaria is a recurring subject that threatens public health globally. There is an urgent need to seek new antimalarial agents. This study seeking new antimalarials from medicinal plants is guided by ethnobotany. Materials and methods: Medicinal plants of the Luo and Kuria ethnic groups of Kenya with high usage reports were screened in vitro for their antiplasmodial activity using the SYBR Green I fluorescence assay (MSF assay). Results: The IC50's for drugs and total plant extracts ranged from 0.01217 to 10.679 mg/ml. Extracts were more active on chloroquine sensitive than resistant Plasmodium falciparum strains. Tylosema fassoglense, Ageratum conyzoides and Ocimum kilimandscharicum exhibited promising results. Plectranthus barbatus did not show activity. Conclusion: Ethnobotanical knowledge was sufficiently reliable for identifying plant extracts with antiplasmodial activity. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

引用

页码：779 / 781

页数：3

共 17 条

[1] Antimalarial Drug Sensitivity Profile of Western Kenya Plasmodium falciparum Field Isolates Determined by a SYBR Green I in vitro Assay and Molecular Analysis [J].

Akala, Hoseah M. ;

Eyase, Fredrick L. ;

Cheruiyot, Agnes C. ;

Omondi, Angela A. ;

Ogutu, Bernhards R. ;

Waters, Norman C. ;

Johnson, Jacob D. ;

Polhemus, Mark E. ;

Schnabel, David C. ;

Walsh, Douglas S. .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2011, 85 (01) :34-41

[2] A search for natural bioactive compounds in Bolivia through a multidisciplinary approach.: Part V.: Evaluation of the antimalarial activity of plants used by the Tacana Indians [J].

Deharo, E ;

Bourdy, G ;

Quenevo, C ;

Muñoz, V ;

Ruiz, G ;

Sauvain, M .

JOURNAL OF ETHNOPHARMACOLOGY, 2001, 77 (01) :91-98

[3] QUANTITATIVE ASSESSMENT OF ANTI-MALARIAL ACTIVITY INVITRO BY A SEMIAUTOMATED MICRODILUTION TECHNIQUE [J].

DESJARDINS, RE ;

CANFIELD, CJ ;

HAYNES, JD ;

CHULAY, JD .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1979, 16 (06) :710-718

[4] Phytochemical constituents of some Nigerian medicinal plants [J].

Edeoga, HO ;

Okwu, DE ;

Mbaebie, BO .

AFRICAN JOURNAL OF BIOTECHNOLOGY, 2005, 4 (07) :685-688

[5] The value of plants used in traditional medicine for drug discovery [J].

Fabricant, DS ;

Farnsworth, NR .

ENVIRONMENTAL HEALTH PERSPECTIVES, 2001, 109 :69-75

[6] Biological evaluation of substituted quinolines [J].

Franck, X ;

Fournet, A ;

Prina, E ;

Mahieux, R ;

Hocquemiller, R ;

Figadère, B .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2004, 14 (14) :3635-3638

[7] Assessment and continued validation of the malaria SYBR green I-based fluorescence assay for use in malaria drug screening [J].

Johnson, Jacob D. ;

Dennull, Richard A. ;

Gerena, Lucia ;

Lopez-Sanchez, Miriam ;

Roncal, Norma E. ;

Waters, Norman C. .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2007, 51 (06) :1926-1933

[8]

Juma P.W., 2011, PHYTOCHEM LETT, V4, P176

[9] In vitro antiplasmodial activity of some plants used in Kisii, Kenya against malaria and their chloroquine potentiation effects [J].

Muregi, FW ;

Chhabra, SC ;

Njagi, ENM ;

Lang'at-Thoruwa, CC ;

Njue, WM ;

Orago, ASS ;

Omar, SA ;

Ndiege, IO .

JOURNAL OF ETHNOPHARMACOLOGY, 2003, 84 (2-3) :235-239

[10] The antiprotozoal activity of methylated flavonoids from Ageratum conyzoides L. [J].

Nour, Amal M. M. ;

Khalid, Sami A. ;

Kaiser, Marcel ;

Brun, Reto ;

Abdalla, Wai'l E. ;

Schmidt, Thomas J. .

JOURNAL OF ETHNOPHARMACOLOGY, 2010, 129 (01) :127-130

← 1 2 →