Characterization of the human CREB3L2 gene promoter

被引：4

作者：

Panagopoulos, Ioannis ^{[1
]}

Mertens, Fredrik ^{[1
]}

机构：

[1] Univ Hosp, Dept Clin Genet, SE-22185 Lund, Sweden

来源：

ONCOLOGY REPORTS | 2009年 / 21卷 / 03期

基金：

瑞典研究理事会;

关键词：

CREB3L2; gene; promoter; CRE binding site; bidirectional; AKR1D1; GRADE FIBROMYXOID SARCOMA; TRANSCRIPTION FACTOR; BIDIRECTIONAL PROMOTERS; BINDING; PATHWAY; REGIONS; BBF2H7; ENZYME; FUSION; GENOME;

D O I：

10.3892/or_00000264

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

CREB3L2 encodes a member of the CREB3 family of transcription factors. We characterized its promoter region, showing that it is asymmetrically bidirectional, also driving the expression of a variant of AKR1D1. It has a CRE binding site which is conserved among mammalians; removal or alteration of it resulted in reduced promoter activity. When transiently transfecting the HEK293 cell line with constructs with partially deleted promoter regions, 5' deletions beyond 1058-bp upstream of the transcription starting site resulted in successive reduction of the activity. The inclusion of the untranslated part of CREB3L2 exon 1 strongly inhibited the promoter activity. Forskolin resulted in a decreased reporter activity, whereas phorbol 12-myristate 13-acetate increased the promoter activity irrespective of the status of the CRE binding site. The presence of the CRE site indicates autoregulation of CREB3L2 and/or regulation via other members of the CREB3 family or a variety of bZIP transcription factors.

引用

页码：615 / 624

页数：10

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