Transient expression of GABAA receptor α2 and α3 subunits in differentiating cerebellar neurons

被引:27
|
作者
Takayama, C [1 ]
Inoue, Y [1 ]
机构
[1] Hokkaido Univ, Sch Med, Dept Mol Neuroanat, Kita Ku, Sapporo, Hokkaido 0608638, Japan
来源
DEVELOPMENTAL BRAIN RESEARCH | 2004年 / 148卷 / 02期
关键词
Purkinje cell; granule cell; cerebellar nucleus; Bergmann glia; in situ hybridization;
D O I
10.1016/j.devbrainres.2003.11.007
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
In the adult mammalian brain, synaptic transmission mediated by gamma-amino butyric acid (GABA) plays a role in inhibition of excitatory synaptic transmission. During brain development, GABA is involved in brain morphogenesis. To clarify how GABA exerts its effect on immature neurons, we examined the expression of the GABA(A) receptor alpha2 and alpha3 subunits, which are abundantly expressed before alpha1 and alpha6 subunits appear, in the developing mouse cerebellum using in situ hybridization. Proliferating neuronal precursors in the ventricular zone and external granular layer expressed neither alpha2 nor alpha3 subunits. Hybridization signals for the alpha2 and alpha3 subunit mRNAs first appeared in the differentiating zone at embryonic day 13 (E13). The alpha2 subunit was detected in the migrating and differentiating granule cells and cerebellar nucleus neurons until postnatal day 14 (P14). Hybridization signals for the alpha2 and alpha3 subunit mRNA, on the other hand, were localized in the developing Purkinje cells and cerebellar nucleus neurons, and disappeared from Purkinje cells by the end of first postnatal week. Taken together, this indicated that the alpha2 and alpha3 subunits were abundantly expressed in distinct types of cerebellar neurons after completing cell proliferation while forming the neural network. These results suggest that GABA might extrasynaptically activate the GABA(A) receptors containing alpha2 and/or alpha3 subunits on the differentiating neurons before finishing the formation of synapses and networks, and could be involved in neuronal differentiation and maturation in the cerebellum. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:169 / 177
页数:9
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