Isolation and inheritance of microsatellite markers in the common carp Cyprinus carpio

Three microsatellite loci, Cca-17*, Cca-21*, and Cca-30*, were isolated from a partial genome library of common carp Cyprinus carpio. Primers for PCR amplification of template DNA were designed based on the unique sequences flanking each motif. The total number of alleles detected from nishikigoi and wild type common carp population were five, six and nine at loci Cca-17*, Cca-21*, and Cca-30*, respectively. Inheritance was shown to be Mendelian by analysis of genotype ratio in Fl offspring of six families of nishikigoi. Allele *69 at locus Cca-21* and allele *266 at locus Cca-30* were detected only in wild type common carp with frequencies of 0.304 and 0.458, respectively, while allele *288 was observed only in the nishikigoi with the frequencies ranged from 0.600-0.610.