KRT14 promoting invasion and migration of lung cancer cells through ROCK-1 signaling pathway

被引:0
作者
Wang, Xiangying
Han, Lina
Shan, Shimin
Sun, Yuxia
Mao, Yimin [1 ]
机构
[1] Henan Univ Sci & Technol, Coll Clin Med, Luoyang 471003, Peoples R China
来源
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY | 2017年 / 10卷 / 01期
关键词
KRT14; lung cancer; ROCK-1; A549; invasion; migration; KINASE INHIBITOR; MARKER; LIVER;
D O I
暂无
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Objective: To explore the effects of KRT14 on the invasion and migration abilities of lung cancer cells and the relevant mechanism. Methods: The expression of KRT14 was measured in different lung cancer cell lines by Western blotting; lentivirus silencing KRT14, GFP fluorescence and Western blotting were used to detect the LV3-KRT14 silencing efficiency and efficacy; the effect of silencing KRT14 on invasion of lung cancer cells was detected by Transwell invasion assay; the effect of silencing KRT14 on migration of lung cancer cells was detected by wound scratch assay; the expression of the proteins ROCK-1 and RhoA was detected by Western blotting; the interaction between KRT14 and RhoA was detected by CoIP assay; the effects of silencing KRT14 on size and volume of lung cancer were detected in the subcutaneous tumor formation experiment in nude mice; and the expression of KRT14 and RhoA was measured by immunohistochemistry in the LV3-NC and LV3-KRT14 groups. Results: The expression of KRT14 was the highest in the A549 cells; LV3-KRT14 lentivirus could effectively inhibit the expression of KRT14; silencing KRT14 inhibited the invasion and migration abilities of the lung cancer A549 cells; silencing KRT14 could down-regulate the expression of ROCK-1 and RhoA; KRT14 directly interacted with RhoA; the tumor volume and weight of tumor-bearing mice decreased significantly in the LV3-KRT14 group as compared to the LV3-NC group; and IHC results showed that compared to the LV3-NC group, the expression of KRT14 and RhoA decreased significantly. Conclusion: KRT14 influences the invasion and migration abilities of lung cancer cells through the RhoA/ROCK-1 signaling pathway.
引用
收藏
页码:795 / 803
页数:9
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