The agro-food sphere in the modern period of time is the sphere whose main goal is the coordination and integration of the actions of various levels of its participants. The agro-food sphere includes three levels of participants: producers of food products, wholesale and retail trade enterprises, and final consumers of food products. Producers of food products, in turn, set themselves the goal of establishing effective logistics channels and further delivery their goods to the retail network through wholesale enterprises of various forms. Wholesale enterprises, using their own distribution centers or using integrated logistic formats with retail trade networks, organize the delivery of the received goods from manufacturers to retail companies of various formats. Retail companies are stationary and non-stationary, accumulating the required assortment of goods on the shelves of stores, meet the needs of key customers who need quality and safe goods at the best prices. These participants in the agro-food sector, in order to increase the efficiency of production and trade activities, and taking into account the interests of end users, form agrological chains that are integrated and have a pronounced synergistic effect. All three levels of participants in the agro-food sphere, due to the active development of both production and trade technologies, require new solutions and tools to improve overall effectiveness. These new tools include digital technologies, which to date reflect the realities and requirements of the concept of the digital economy of Russia as a whole. The publication examines the modern experience of using digital technologies in the activities of participants in all three levels in the agro-food sphere, as well as the possible positive and negative consequences from their implementation in the activities of agro-food sector participants.