Microarray-guided discovery of two-photon (2P) small molecule probes for live-cell imaging of cysteinyl cathepsin activities

被引：20

作者：

Na, Zhenkun ^{[1
]}

Li, Lin ^{[1
]}

Uttamchandani, Mahesh ^{[1
,2
]}

Yao, Shao Q. ^{[1
]}

机构：

[1] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore

[2] Def Med & Environm Res Inst, DSO Natl Labs, Singapore 117510, Singapore

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 58期

关键词：

PROTEASES;

D O I：

10.1039/c2cc33476c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A microarray immobilized with 105 aldehyde-containing small molecules was screened against mammalian cell lysates over-expressing cathepsin L to identify two potent inhibitors, which were subsequently converted into cell-permeable probes capable of live-cell imaging of endogenous cysteinyl cathepsin activities by two-photon fluorescence microscopy.

引用

页码：7304 / 7306

页数：3

共 2 条

[1] Silafluorene as a promising core for cell-permeant, highly bright and two-photon excitable fluorescent probes for live-cell imaging
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DYES AND PIGMENTS, 2021, 187 (187)
[2] Water-soluble small-molecule probes for RNA based on a two-photon fluorescence "off-on'' process: systematic analysis in live cell imaging and understanding of structure-activity relationships
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CHEMICAL COMMUNICATIONS, 2017, 53 (99) : 13245 - 13248

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