Control of specific gene expression by gibberellin and brassinosteroid

被引:114
|
作者
Bouquin, T [1 ]
Meier, C [1 ]
Foster, R [1 ]
Nielsen, ME [1 ]
Mundy, J [1 ]
机构
[1] Univ Copenhagen, Inst Mol Biol, Dept Plant Physiol, DK-1353 Copenhagen K, Denmark
关键词
D O I
10.1104/pp.010173
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We identified a recessive, brassinolide-insensitive mutant caused by a deletion allele (bri1-201) of the brassinosteroid (BR) receptor BRI1. The bri1-201 mutant displayed altered expression levels of genes differentially regulated by gibberellin (GA). RNA-blot analysis revealed that BR and GA antagonistically regulate the accumulation of mRNAs of the GA-responsive GASA1 gene, as well as the GA-repressible GA5 gene. Expression studies with cycloheximide indicated that the antagonistic effects of GA and BR on GA5 require de novo protein synthesis. Reporter transgene analyses and RNA-blot analysis showed that BR and GA modulate GA5 expression, at least in part, at the transcriptional level, and that the signals are independent and subtractive.
引用
收藏
页码:450 / 458
页数:9
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