Longitudinal Tracking of Human Fetal Cells Labeled with Super Paramagnetic Iron Oxide Nanoparticles in the Brain of Mice with Motor Neuron Disease

被引：28

作者：

Bigini, Paolo ^{[1
]}

Diana, Valentina ^{[8
,9
]}

Barbera, Sara ^{[1
]}

Fumagalli, Elena ^{[1
]}

Micotti, Edoardo ^{[1
]}

Sitia, Leopoldo ^{[1
]}

Paladini, Alessandra ^{[1
]}

Bisighini, Cinzia ^{[1
]}

De Grada, Laura ^{[2
]}

Coloca, Laura ^{[2
]}

Colombo, Laura ^{[1
]}

Manca, Pina ^{[2
]}

Bossolasco, Patrizia ^{[3
]}

Malvestiti, Francesca ^{[2
]}

Fiordaliso, Fabio ^{[1
]}

Forloni, Gianluigi ^{[1
]}

Morbidelli, Massimo ^{[4
]}

Salmona, Mario ^{[1
]}

Giardino, Daniela ^{[2
]}

Mennini, Tiziana ^{[1
]}

Moscatelli, Davide ^{[5
]}

Silani, Vincenzo ^{[6
,7
,8
,9
]}

Cova, Lidia ^{[8
,9
]}

机构：

[1] Mario Negri Inst Pharmacol Res, Milan, Italy

[2] ISCCR Ist Auxol Italiano, Lab Citogenet, Cusano Milanino, Italy

[3] Univ Milan, Dipartimento Farmacol Chemioterapia & Tossicol Me, Fdn Matarelli, Milan, Italy

[4] ETH, Inst Chem & Bioengn, Zurich, Switzerland

[5] Politecn Milan, Dipartimento Chim Mat & Ingn Chim G Natta, I-20133 Milan, Italy

[6] Univ Milan, Dept Neurol, Ctr Dino Ferrari, IRCCS Ist Auxol Italiano, Milan, Italy

[7] Univ Milan, Neurosci Lab, Ctr Dino Ferrari, IRCCS Ist Auxol Italiano, Milan, Italy

[8] IRCCS Ist Auxol Italiano, Neurosci Lab, Cusano Milanino, Italy

[9] IRCCS Ist Auxol Italiano, Dept Neurol, Cusano Milanino, Italy

来源：

PLOS ONE | 2012年 / 7卷 / 02期

关键词：

STEM-CELLS; TRANSFECTION AGENT; MOUSE MODEL; IN-VIVO; SCLEROSIS; CORD; DIFFERENTIATION; TRANSPLANTATION; EXPRESSION;

D O I：

10.1371/journal.pone.0032326

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Stem Cell (SC) therapy is one of the most promising approaches for the treatment of Amyotrophic Lateral Sclerosis (ALS). Here we employed Super Paramagnetic Iron Oxide nanoparticles (SPIOn) and Hoechst 33258 to track human Amniotic Fluid Cells (hAFCs) after transplantation in the lateral ventricles of wobbler (a murine model of ALS) and healthy mice. By in vitro, in vivo and ex vivo approaches we found that: 1) the main physical parameters of SPIOn were maintained over time; 2) hAFCs efficiently internalized SPIOn into the cytoplasm while Hoechst 33258 labeled nuclei; 3) SPIOn internalization did not alter survival, cell cycle, proliferation, metabolism and phenotype of hAFCs; 4) after transplantation hAFCs rapidly spread to the whole ventricular system, but did not migrate into the brain parenchyma; 5) hAFCs survived for a long time in the ventricles of both wobbler and healthy mice; 6) the transplantation of double-labeled hAFCs did not influence mice survival.

引用

页数：12

共 37 条

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