Detection of Coxiella burnetii DNA in the environment during and after a large Q fever epidemic in the Netherlands

被引：36

作者：

de Bruin, A. ^{[1
]}

Janse, I. ^{[1
]}

Koning, M. ^{[1
]}

de Heer, L. ^{[1
]}

van der Plaats, R. Q. J. ^{[1
]}

van Leuken, J. P. G. ^{[1
]}

van Rotterdam, B. J. ^{[1
]}

机构：

[1] Natl Inst Publ Hlth & Environm, NL-3720 BA Bilthoven, Netherlands

来源：

JOURNAL OF APPLIED MICROBIOLOGY | 2013年 / 114卷 / 05期

关键词：

detection; molecular epidemiology; polymerase chain reaction (PCR); veterinary; DUST SAMPLES; OUTBREAK; FARMS; PCR; INFORMATION; EXTRACTION; BARN;

D O I：

10.1111/jam.12163

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aim To investigate the Coxiella burnetii DNA content in environmental samples that may contribute to the transmission of C.burnetii. Methods and Results During a large Q fever outbreak in the Netherlands, surface swabs and aerosol samples were collected inside stables and around six Q fever-affected ruminant farms, which are located in municipalities varying in Q fever incidence. After the outbreak in 2010, aerosol samples were collected in the same geographical areas. The use of an optimized multiplex qPCR for the detection of C.burnetii DNA revealed that all samples obtained inside stables were positive. In addition, the C.burnetii DNA content in aerosol samples collected in stables is significantly higher than in aerosol samples collected around the farms. Finally, the C.burnetii DNA content in aerosol samples collected in the same geographical locations was lower in 2010 in comparison with 2009. Conclusions The reduction in C.burnetii DNA content in aerosol samples between 2009 and 2010 is in agreement with the reduction in Q fever incidence in the same geographical areas. Significance and Impact of the Study The presence of C.burnetii DNA in environmental samples collected on and around ruminant farms supports the hypothesis that C.burnetii can be disseminated from ruminant farms to the surrounding areas.

引用

页码：1395 / 1404

页数：10

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