Relationship between the expression of Rab family GTPases and neuropeptide hormones in the brain of Bombyx mori

被引：16

作者：

Uno, Tomohide ^{[1
]}

Sakamoto, Kazuki ^{[1
]}

Isoyama, Yuri ^{[1
]}

Hiragaki, Susumu ^{[2
]}

Uno, Yuichi ^{[3
]}

Kanamaru, Kengo ^{[1
]}

Yamagata, Hiroshi ^{[1
]}

Takagi, Michihiro ^{[4
]}

Mizoguchi, Akira ^{[5
]}

Takeda, Makio ^{[2
]}

机构：

[1] Kobe Univ, Fac Agr, Dept Biofunct Chem, Lab Biol Chem,Nada Ku, Kobe, Hyogo 6578501, Japan

[2] Kobe Univ, Grad Sch Agr Sci, Nada Ku, Kobe, Hyogo 6578501, Japan

[3] Kobe Univ, Dept Plant Resource Sci, Fac Agr, Nada Ku, Kobe, Hyogo 6578501, Japan

[4] Natl Inst Anim Hlth, Res Team Viral Dis, Tsukuba, Ibaraki 3050856, Japan

[5] Nagoya Univ, Grad Sch Sci, Div Biol Sci, Chikusa Ku, Nagoya, Aichi 4648602, Japan

来源：

HISTOCHEMISTRY AND CELL BIOLOGY | 2013年 / 139卷 / 02期

基金：

日本学术振兴会;

关键词：

Bombyx mori; Rab; Brain; Eclosion hormone; Insect; Bombyxin; INSULIN-RELATED PEPTIDE; AMINO-ACID-SEQUENCE; WING IMAGINAL DISKS; MOTH MANDUCA-SEXTA; PROTHORACICOTROPIC HORMONE; ECLOSION HORMONE; LEPIDOPTERA BOMBYCIDAE; HELICOVERPA-ARMIGERA; NEUROSECRETORY-CELLS; VESICULAR TRANSPORT;

D O I：

10.1007/s00418-012-1021-5

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Rab proteins are small GTPases that play essential roles in vesicle transport. In this study, we examined the expression of Rab proteins and neuropeptide hormones in the brain of the silkworm, Bombyx mori. We produced antibodies against B. mori Rab1 and Rab14 in rabbits. Immunoblotting of samples of brain tissue from B. mori revealed a single band for each antibody. Rab1 and Rab14 immunohistochemical labeling in the brain of B. mori was restricted to neurons of the pars intercerebralis and dorsolateral protocerebrum. Rab1, Rab7 and Rab14 co-localized with bombyxin. Rab1 and Rab7 co-localized with eclosion hormone. Rab1 co-localized with prothoracicotropic hormone. These results suggest that Rab1, Rab7 and Rab14 may be involved in neuropeptide transport in the brain of B. mori. This is the first report on the specificity of Rab proteins for the secretion of different neuropeptides in insects.

引用

页码：299 / 308

页数：10

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