Interleukin-17 and Interleukin-22 Induced Proinflammatory Cytokine Production in Keratinocytes via Inhibitor of Nuclear Factor κ B Kinase-α Expression

被引:25
作者
Cho, Kyung-Ah [1 ]
Kim, Jin-Young [1 ]
Woo, So-Youn [1 ]
Park, Hyun Jeong [2 ]
Lee, Kyung Ho [2 ]
Pae, Chi-Un [3 ]
机构
[1] Ewha Womans Univ, Sch Med, Dept Microbiol, Seoul, South Korea
[2] Catholic Univ Korea, Coll Med, Dept Dermatol, Seoul, South Korea
[3] Catholic Univ Korea, Coll Med, Dept Psychiat, Seoul, South Korea
关键词
Cell differentiation; IKK alpha; Inflammation; Interleukin-17; Interleukin-22; Keratinocytes; IKK-ALPHA; DIFFERENTIATION; EPIDERMIS; IL-22; INFLAMMATION;
D O I
10.5021/ad.2012.24.4.398
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Background: The pathogenesis of psoriasis may involve the interleukin (IL)-23 and Th17-mediated immune responses. Th17 cells secret IL-17 and IL-22, which mediates dermal inflammation and acanthosis. Objective: As inhibitor of nuclear factor kappa B kinase-alpha (IKK alpha) has been previously identified as a primary regulator of keratinocyte differentiation and proliferation, we proposed that IL-17 and IL-22 might affect keratinocyte differentiation by changing the expression of IKK alpha. Methods: We employed HaCaT cells maintained culture medium at a low calcium concentration (0.06 mM) and induced differentiation by switching to the high concentration (2.8 mM) media with IL-17 or IL-22, then compared the IKK alpha expression and the cell cycle. We employed reconstituted human epidermal skin (Neoderm) and mice ears for the in vivo studies. Results: Elevated calcium concentration induced IKK alpha expression and terminal differentiation with cell cycle arrest in HaCaT cell cultures. Moreover, IL-17 and IL-22 treatment also induced IKK alpha in HaCaT cells and reconstituted human epidermis. IKK alpha induction was also noted, following the injection of IL-17 and IL-22 into mice ears. Conclusion: Although the induction of IKK alpha was accompanied by keratinocyte differentiation, IL-17 and IL-22 did not affect calcium-mediated differentiation or the cell cycle. Rather, IL-17 and IL-22 appear to contribute to the inflammation occurring via the induction of IKK alpha from keratinocytes or skin layers. (Ann Dermatol 24(4) 398 similar to 405, 2012)
引用
收藏
页码:398 / 405
页数:8
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