Effect of sequential medium on in vitro culture of goat ovarian cortical tissue

被引：13
|
作者：
Magalhaes-Padilha, D. M. [1 ]
Andrade, P. M. [1 ]
Sales, E. T. [1 ]
Araujo, V. R. [1 ]
Lima, I. M. T. [1 ]
Castro, S. V. [1 ]
Faustino, L. R. [1 ]
Lopes, C. A. P. [1 ]
Campello, C. C. [1 ]
Bao, S. N. [2 ]
Gastal, M. O. [3 ]
Gastal, E. L. [3 ]
Figueiredo, J. R. [1 ]
机构：
[1] UECE, Fac Vet, PPGCV, Lab Manipulacao Oocitos & Foliculos Preantrais LA, BR-60740930 Forlaleza, CE, Brazil
[2] Univ Brasilia, Dept Cell Biol, Electron Microscopy Lab, BR-70910900 Brasilia, DF, Brazil
[3] So Illinois Univ, Dept Anim Sci Food & Nutr, Carbondale, IL 62901 USA
来源：
ANIMAL REPRODUCTION SCIENCE | 2012年 / 132卷 / 3-4期
关键词：
Caprine; Folliculogenesis; FSH; GH; Preantral follicle; FOLLICLE-STIMULATING-HORMONE; RECEPTOR GENE-EXPRESSION; GROWTH-HORMONE; PREANTRAL FOLLICLES; MESSENGER-RNA; PRIMORDIAL FOLLICLES; GRANULOSA-CELLS; KIT-LIGAND; OOCYTES; LOCALIZATION;
D O I：
10.1016/j.anireprosci.2012.05.017
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
A sequential medium was evaluated on the survival, activation and growth rates of caprine preantral follicles submitted to a long-term culture period, aiming to establish an ideal in vitro culture system. Ovarian fragments were cultured for 16 days in alpha-MEM+ alone or supplemented with hormones (GH and/or FSH) added sequentially on different days of culture. Ovarian fragments were cultured in the first (days 0-8) and second (days 8-16) halves of the culture period, generating 10 treatments: alpha-MEM+/alpha-MEM+, FSH/FSH, FSH/GH, FSH/FSH + GH, GH/GH, GH/FSH, GH/FSH + GH, FSH + GH/FSH + GH, FSH + GH/FSH and FSH + GH/GH. Follicle morphology, viability and ultrastructure were analyzed. After day 1 of culture. FSH treatments maintained the percentage of normal follicles similar to the fresh control. At day 16 of culture, the treatment FSH/GH showed the highest (P< 0.05) percentage of normal follicles. The ultrastructure of follicles was preserved in the fresh control and FSH/GH treatment. Follicles cultured with FSH/GH had a higher (P< 0.05) viability than alpha-MEM+; however the viability was lower (P< 0.05) when compared to the fresh control. The FSH/GH treatment showed the highest (P< 0.05) percentage of follicular activation and secondary follicle formation and produced the largest (P< 0.05) mean follicular diameter after 16 days of culture. In conclusion, a sequential medium supplemented with FSH followed by GH during a long-term culture maintains the survival, viability and ultrastructure of goat preantral follicles, and promotes activation and secondary follicles. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：159 / 168
页数：10
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