Assessment of mercury chloride-induced toxicity and the relevance of P2X7 receptor activation in zebrafish larvae

被引：19

作者：

Cruz, Fernanda Fernandes ^{[1
,2
]}

Leite, Carlos Eduardo ^{[1
,3
]}

Brandao Pereira, Talita Carneiro ^{[5
]}

Bogo, Mauricio Reis ^{[4
,5
]}

Bonan, Carla Denise ^{[4
]}

Oliveira Battastini, Ana Maria ^{[3
,6
]}

Campos, Maria Martha ^{[1
,7
]}

Morrone, Fernanda Bueno ^{[1
,2
]}

机构：

[1] Pontificia Univ Catolica Rio Grande do Sul, Inst Toxicol & Farmacol, BR-90619900 Porto Alegre, RS, Brazil

[2] Pontificia Univ Catolica Rio Grande do Sul, Fac Farm, BR-90619900 Porto Alegre, RS, Brazil

[3] Univ Fed Rio Grande do Sul, Programa Posgrad Ciencias Med, BR-90035003 Porto Alegre, RS, Brazil

[4] Pontificia Univ Catolica Rio Grande do Sul, Fac Biociencias, BR-90619900 Porto Alegre, RS, Brazil

[5] Pontificia Univ Catolica Rio Grande do Sul, Lab Biol Genom & Mol, BR-90619900 Porto Alegre, RS, Brazil

[6] Univ Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Dept Bioquim, Porto Alegre, RS, Brazil

[7] Pontificia Univ Catolica Rio Grande do Sul, Fac Odontol, BR-90619900 Porto Alegre, RS, Brazil

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY | 2013年 / 158卷 / 03期

关键词：

P2X7R; Mercury; Zebrafish larvae; Oxidative stress; IN-VIVO; OXIDATIVE STRESS; P2X(7); ATP; EXPRESSION; ENVIRONMENT; SECRETION; INSIGHTS; METALS; BRAIN;

D O I：

10.1016/j.cbpc.2013.07.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Zebrafish (Danio rerio) has been adopted as a model for behavioral, immunological and toxicological studies. Mercury is a toxic heavy metal released into the environment. There is evidence indicating that heavy metals can modulate ionotropic receptors, including the purinergic receptor P2X7. Therefore, this study evaluated the in vivo effects of acute exposure to mercury chloride (HgCl2) in zebrafish larvae and to investigate the involvement of P2X7R in mercury-related toxicity. Larvae survival was evaluated for 24 h after exposure to HgCl2. ATP or A740003. The combination of ATP (1 mM) and HgCl2 (20 mu g/L) decreased survival when compared to ATP 1 mM. The antagonist A740003 (300 and 500 nM) increased the survival time, and reversed the mortality caused by ATP and HgCl2 in association. Quantitative real time PCR showed a decrease of P2X7R expression in the larvae treated with HgCl2 (20 mu g/L). Evaluating the oxidative stress our results showed decreased CAT (catalase) activity and increased MDA (malondialdehyde) levels. Of note, the combination of ATP with HgCl2 showed an additive effect. This study provides novel evidence on the possible mechanisms underlying the toxicity induced by mercury, indicating that it is able to modulate P2X7R in zebrafish larvae. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：159 / 164

页数：6