Prevalence and molecular characterization of porcine enteric caliciviruses and first detection of porcine kobuviruses in US swine

被引:34
作者
Sisay, Zufan [1 ,2 ]
Wang, Qiuhong [1 ]
Oka, Tomoichiro [1 ]
Saif, Linda [1 ]
机构
[1] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Vet Prevent Med, Food Anim Hlth Res Program, Wooster, OH 44691 USA
[2] Univ Addis Ababa, Dept Microbiol & Immunol, Aklilu Lemma Inst Pathobiol, Addis Ababa, Ethiopia
基金
美国国家卫生研究院;
关键词
REVERSE TRANSCRIPTION-PCR; GENETIC DIVERSITY; HUMAN SAPOVIRUSES; NOROVIRUSES; JAPAN; PIGS; RECOMBINATION; DIARRHEA; PRIMERS; VIRUSES;
D O I
10.1007/s00705-013-1619-5
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The prevalence of porcine sapoviruses (SaVs) and noroviruses (NoVs) in nursing piglets on three pig farms in Ohio was studied. Fecal samples (n = 139) were collected from individual pigs and screened for caliciviruses by RT-PCR. Phylogenetic analysis was conducted using partial sequences of the RNA polymerase region. Three different SaV genogroups, including a newly emerging one (DO19 Korea-like) were detected. No NoVs were detected. Kobuviruses, emerging members of the family Picornaviridae, were detected by primers designed for SaV. To our knowledge, this is the first report of porcine DO19 Korea-like SaV and kobuvirus in the United States.
引用
收藏
页码:1583 / 1588
页数:6
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