Single microspore reverse transcription-polymerase chain reaction: Dynamic change of gene expression during microsporogenesis

被引：2

作者：

Zhou, B

Sauter, M

Sun, MX ^{[1
]}

机构：

[1] Wuhan Univ, Coll Life Sci, Key Lab MOE Plant Dev Biol, Wuhan 430072, Peoples R China

[2] Univ Kiel, Inst Bot, D-24098 Kiel, Germany

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2005年 / 69卷 / 11期

基金：

中国国家自然科学基金;

关键词：

Oryza sativa L; microspores; single microspore (RT-PCR); gene expression dynamics;

D O I：

10.1271/bbb.69.2240

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive and reliable single microspore reverse transcription-polymerase chain reaction (RT-PCR) assay was developed that requires little sample material and only a short sampling time and ensures template preservation. We propose that the assay provides a useful tool to study not only cell-cycle related events in developing pollen but also the molecular mechanisms underlying male sterility and mutants impaired in pollen development.

引用

收藏

页码：2240 / 2243

页数：4

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