Anaerobic ammonia oxidation with nitrogen dioxide by Nitrosomonas eutropha

被引:187
作者
Schmidt, I [1 ]
Bock, E [1 ]
机构
[1] UNIV HAMBURG,INST ALLGEMEINE BOT,ABT MIKROBIOL,D-22609 HAMBURG,GERMANY
关键词
Nitrosomonas; anaerobic ammonia oxidation; anaerobic cell growth; nitrogen dioxide; nitric oxide; nitrous oxide; dinitrogen;
D O I
10.1007/s002030050422
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Nitrosomonas eutropha, an obligately lithoautotrophic bacterium, was able to nitrify and denitrify simultaneously under anoxic conditions when gaseous nitrogen dioxide (NO2) was supplemented to the atmosphere. In the presence of gaseous NO2, ammonia was oxidized, nitrite and nitric oxide (NO) were formed, and hydroxylamine occurred as an intermediate. Between 40 and 60% of the produced nitrite was denitrified to dinitrogen (N-2). Nitrous oxide (N2O) was shown to be an intermediate of denitrification. Under an N-2 atmosphere supplemented with 25 ppm NO2 and 300 ppm CO2, the amount of cell protein increased by 0.8 7 mg protein per mmol ammonia oxidized, and the cell number of N. eutropha increased by 5.8 x 10(9) cells per mmol ammonia oxidized. In addition, the ATP and NADH content increased by 4.3 mu mol ATP (g protein)(-1) and 6.3 mu mol NADH (g protein)(-1) and was about the same in both anaerobically and aerobically grown cells. Without NO2, the ATP content decreased by 0.7 mu mol (g protein)(-1), and the NADH content decreased by 1.2 mu mol (g protein)(-1). NO was shown to inhibit anaerobic ammonia oxidation.
引用
收藏
页码:106 / 111
页数:6
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