Caffeic Acid Phenethyl Ester Inhibits Endothelial Tissue Factor Expression

被引：7

作者：

Gebhard, Catherine ^{[1
,2
,3
]}

Staehli, Barbara Elisabeth ^{[1
,2
,3
]}

Largiader, Stephanie ^{[1
,2
]}

Holy, Erik Walter ^{[1
,2
,3
]}

Akhmedov, Alexander ^{[1
,2
]}

Camici, Giovanni Guido ^{[1
,2
]}

Luescher, Thomas Felix ^{[1
,2
,3
]}

Tanner, Felix Christoph ^{[1
,2
,3
]}

机构：

[1] Univ Zurich, Inst Physiol, CH-8057 Zurich, Switzerland

[2] Univ Zurich, Ctr Integrat Human Physiol, CH-8057 Zurich, Switzerland

[3] Univ Zurich Hosp, Ctr Cardiovasc, CH-8057 Zurich, Switzerland

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2013年 / 36卷 / 06期

基金：

瑞士国家科学基金会;

关键词：

coagulation; thrombosis; endothelial function; ATHEROSCLEROSIS; ACTIVATION; PATHWAY;

D O I：

10.1248/bpb.b12-01039

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Caffeic acid phenethyl ester (CAPE) is a component of honeybee hives with various beneficial properties. Tissue factor (TF), the key trigger of thrombosis, is expressed in human endothelial cells. This study was designed to investigate whether CAPE modulates TF expression in human aortic endothelial cells (HAECs). Western blots and real-time polymerase chain reactions were performed. CAPE (10(-7)-10(-5)M) inhibited tumor necrosis factor (TNF)-alpha induced endothelial TF protein expression by 2.1-fold at 10(-5)M (p<0.0001). Similarly, TF surface activity was reduced (p<0.02). In contrast, TF mRNA expression, TF promoter activity, and mitogen-activated protein (MAP) kinase activation remained unaltered. In conclusion, CAPE inhibits TF protein expression and activity at the posttranscriptional level thereby exhibiting anti-thrombotic potential.

引用

页码：1032 / 1035

页数：4

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