Histological and biomechanical properties of regenerated articular cartilage using chondrogenic bone marrow stromal cells with a PLGA scaffold in vivo

被引：45

作者：

Han, Seung Hwan ^{[2
]}

Kim, Yun Hee ^{[1
,3
]}

Park, Min Sung ^{[1
,3
]}

Kim, In Ae ^{[4
]}

Shin, Jung Woog ^{[4
]}

Yangs, Woo Ick ^{[5
]}

Jee, Kyoung Soo ^{[6
]}

Park, Ki Dong ^{[6
]}

Ryu, Gyu Ha ^{[7
]}

Lee, Jin Woo ^{[1
,3
]}

机构：

[1] Yonsei Univ, Coll Med, Dept Orthopaed Surg 29, Seoul 120752, South Korea

[2] Ajou Univ, Sch Med, Dept Orthopaed Surg, Suwon 442749, South Korea

[3] Yonsei Univ, Coll Med, Brain Korea Project Med Sci 21, Seoul 120752, South Korea

[4] Inje Univ, Coll Biomed Sci & Engn, Dept Biomed Engn, Kimhae 621749, South Korea

[5] Yonsei Univ, Coll Med, Dept Pathol, Seoul 120752, South Korea

[6] Ajou Univ, Dept Mol Sci & Technol, Suwon 442749, South Korea

[7] Korea Food & Drug Adm, Dept Med Devices & Radiat Hlth, Div Med Device Stand, Seoul 122704, South Korea

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2008年 / 87A卷 / 04期

关键词：

bone marrow-derived mesenchymal stem cells; poly lactic-co-glycolic acid; cartilage; cartilage tissue engineering;

D O I：

10.1002/jbm.a.31828

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The properties of regenerated cartilage using bone marrow-derived mesenchymal stem cells (MSCs) and poly lactic-co-glycolic acid (PLGA) scaffold composites pre-treated with TGF-beta 3 were investigated and compared to the non-TGF-beta 3 treated MSCs/PLGA composites in a rabbit model. We prepared MSCs/PLGA scaffold composites and pretreated it with TGF-beta 3 for 3 weeks prior to transplantation. Then, composites were transplanted to the osteochondral defect in the rabbit knee. After 12 weeks of transplantation, 10 of the 12 rabbits in which TGF-beta 3 pretreated MSCs/PLGA scaffold composites were transplanted showed cartilaginous regeneration. In gross morphology, regenerated cartilage showed smooth, flush, and transparent features. In indentation test, this had about 80% of Young's modulus of normal articular cartilage. Histological examination demonstrated hyaline like cartilage structures with glycosaminoglycan and type II collagen expression. Histological scores were not statistically different to the normal articular cartilage. These results showed improvement of cartilage regeneration compared to the non-TGF-beta 3 pretreated MSCs/PLGA scaffold composite transplanted group. Thus, we have successfully regenerated improved hyaline-like cartilage and determined the feasibility of treating damaged articular cartilage using MSCs/PLGA scaffold composite pretreated with TGF-beta 3. Also, we suggest this treatment modality as another concept of cartilage tissue engineering. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 87A: 850-861, 2008

引用

页码：850 / 861

页数：12

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