Dynamic DNA contacts observed in the NMR structure of winged helix protein-DNA complex

被引：105

作者：

Jin, CW ^{[1
]}

Marsden, I ^{[1
]}

Chen, XQ ^{[1
]}

Liao, XB ^{[1
]}

机构：

[1] Univ Illinois, Coll Med, Dept Biochem & Mol Biol, Chicago, IL 60612 USA

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1999年 / 289卷 / 04期

关键词：

NMR; structure; dynamics; Genesis; winged helix protein;

D O I：

10.1006/jmbi.1999.2819

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genesis is an HNF-3/fkh homologous protein. By using multi-dimensional NMR techniques, we have obtained the solution structure and backbone dynamics of Genesis complexed with a 17 base-pair DNA. Our results indicate that both the local folding and dynamic properties of Genesis are perturbed when it binds to the DNA site. Our data show that a conserved flexible amino acid sequence (wing 1) makes dynamic contacts to DNA in the complex and a short helix is induced by Genesis-DNA interactions. Our data indicate that, unlike the HNF-3 gamma/DNA complex, a magnesium ion is not required in forming the stable Genesis-DNA complex. (C) 1999 Academic Press.

引用

页码：683 / 690

页数：8

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