Dynamic DNA contacts observed in the NMR structure of winged helix protein-DNA complex

被引:105
作者
Jin, CW [1 ]
Marsden, I [1 ]
Chen, XQ [1 ]
Liao, XB [1 ]
机构
[1] Univ Illinois, Coll Med, Dept Biochem & Mol Biol, Chicago, IL 60612 USA
关键词
NMR; structure; dynamics; Genesis; winged helix protein;
D O I
10.1006/jmbi.1999.2819
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Genesis is an HNF-3/fkh homologous protein. By using multi-dimensional NMR techniques, we have obtained the solution structure and backbone dynamics of Genesis complexed with a 17 base-pair DNA. Our results indicate that both the local folding and dynamic properties of Genesis are perturbed when it binds to the DNA site. Our data show that a conserved flexible amino acid sequence (wing 1) makes dynamic contacts to DNA in the complex and a short helix is induced by Genesis-DNA interactions. Our data indicate that, unlike the HNF-3 gamma/DNA complex, a magnesium ion is not required in forming the stable Genesis-DNA complex. (C) 1999 Academic Press.
引用
收藏
页码:683 / 690
页数:8
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