An automated, pressure-driven sampling device for harvesting from liquid cultures for genomic and biochemical analyses

被引：3

作者：

Aragon, Anthony D.

Quinones, Gabriel A.

Allen, Chris

Thomas, Jason

Roy, Sushmita

Davidson, George S.

Wentzell, Peter D.

Millier, Brian

Jaetao, Jason E.

Rodriguez, Angelina L.

Werrier-Washburne, Margaret ^{[1
]}

机构：

[1] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA

[2] Univ New Mexico, Dept Comp Sci, Albuquerque, NM 87131 USA

[3] Sandia Natl Labs, Albuquerque, NM 87185 USA

[4] Dalhousie Univ, Dept Chem, Halifax, NS B3H 4J3, Canada

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2006年 / 65卷 / 02期

关键词：

transcriptional profiling; time course analysis; microarray; yeast; rapid sampling;

D O I：

10.1016/j.mimet.2005.08.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we describe an automated, pressure-driven, sampling device for harvesting 10 to 30 ml samples, in replicate, with intervals as short as 10 s. Correlation between biological replicate time courses measured by microarrays was extremely high. The sampler enables sampling at intervals within the range of many important biological processes. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：357 / 360

页数：4

共 5 条

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