Dissemination of CTX-M-type β-lactamases among clinical isolates of Enterobacteriaceae in Paris, France

被引:255
作者
Eckert, C
Gautier, V
Saladin-Allard, M
Hidri, N
Verdet, C
Ould-Hocine, Z
Barnaud, G
Delisle, F
Rossier, A
Lambert, T
Philippon, A
Arlet, G
机构
[1] Hop Tenon, Serv Bacteriol Hyg, AP HP, F-75970 Paris 20, France
[2] Univ Paris 06, Bacteriol Lab, UPRES EA 2392, Paris, France
[3] Hop St Michel, Serv Bacteriol, Paris, France
[4] CHU Cochin, Serv Bactriol, AP HP, Paris, France
[5] Hop Robert Ballanger, Serv Biol, Aulnay Sous Bois, France
[6] Hop Louis Mourier, Microbiol Serv, AP HP, F-92701 Colombes, France
关键词
D O I
10.1128/AAC.48.4.1249-1255.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We analyzed 19 clinical isolates of the family Enterobacteriaceae (16 Escherichia coli isolates and 3 Klebsiella pneumoniae isolates) collected from four different hospitals in Paris, France, from 2000 to 2002. These strains had a particular extended-spectrum cephalosporin resistance profile characterized by a higher level of resistance to cefotaxime and aztreonam than to ceftazidime. The bla(CTX-M) genes encoding these beta-lactamases were involved in this resistance, with a predominance of bla(CTX-M-15). Ten of the 19 isolates produced both TEM-1- and CTX-M-type enzymes. One strain (E. coli TN13) expressed CMY-2, TEM-1, and CTX-M-14. bla(CTX-M) genes were found on large plasmids. In 15 cases the same insertion sequence, ISEcp1, was located upstream of the 5' end of the bla(CTX-M) gene. In one case we identified an insertion sequence designated IS26. Examination of the other three bla(CTX-M) genes by cloning, sequencing, and PCR analysis revealed the presence of a complex sul1-type integron that includes open reading frame ORF513, which carries the bla gene and the surrounding DNA. Five isolates had the same plasmid DNA fingerprint, suggesting clonal dissemination of CTX-M-15-producing strains in the Paris area.
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页码:1249 / 1255
页数:7
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