Shift in polyphenol profile and sublethal phenotype caused by silencing of anthocyanidin synthase in apple (Malus sp.)

被引：55

作者：

Szankowski, Iris ^{[2
]}

Flachowsky, Henryk ^{[3
]}

Li, Houhua ^{[2
]}

Halbwirth, Heidrun ^{[4
]}

Treutter, Dieter ^{[5
]}

Regos, Ionela ^{[5
]}

Hanke, Magda-Viola ^{[3
]}

Stich, Karl ^{[4
]}

Fischer, Thilo C. ^{[1
]}

机构：

[1] Ludwig Maximilians Univ Munchen, D-82152 Planegg Martinsried, Germany

[2] Leibniz Univ Hannover, Fruit Sci Sect, Inst Biol Prod Syst, D-30419 Hannover, Germany

[3] Inst Breeding Res Hort & Fruit Crops, Fed Res Ctr Cultivated Plants, Julius Kuhn Inst, D-01326 Dresden, Germany

[4] Vienna Univ Technol, Inst Chem Engn, A-1060 Vienna, Austria

[5] Tech Univ Munich, Dept Plant Sci, Unit Fruit Sci, D-85350 Freising Weihenstephan, Germany

来源：

PLANTA | 2009年 / 229卷 / 03期

关键词：

Anthocyanidin synthase; Epicatechin; Flavonoids; Gene silencing; Malus; Transformation; MYB TRANSCRIPTION FACTOR; RED COLORATION; FLAVONOID BIOSYNTHESIS; DOMESTICA BORKH; FRUIT-QUALITY; SKIN COLOR; GENE; IDENTIFICATION; PROANTHOCYANIDINS; PROCYANIDINS;

D O I：

10.1007/s00425-008-0864-4

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We have investigated the consequences of blocking anthocyanin biosynthesis by silencing a key enzyme, anthocyanidin synthase, in transgenic plants of a red-leaved apple cultivar. This is complementary to a previous study of induction of anthocyanin biosynthesis by overexpressing a heterologous transcription factor. Analysis of these opposite phenotypes allows one to study anthocyanin functions in apple and to test the influence of the genetic manipulation on other, related metabolites. As expected, anthocyanin biosynthesis was almost completely blocked and this was accompanied by a shift in the profile of flavonoids and related polyphenols. Most interestingly, a rise in epicatechin was found. A severe reduction of viability by necrotic leaf lesions was also observed, suggesting an essential function of anthocyanins in apple.

引用

页码：681 / 692

页数：12

共 41 条

[1] MYB transcription factors that colour our fruit [J].

Allan, Andrew C. ;

Hellens, Roger P. ;

Laing, William A. .

TRENDS IN PLANT SCIENCE, 2008, 13 (03) :99-102

[2] Isolation and functional analysis of a MYB transcription factor gene that is a key regulator for the development of red coloration in apple skin [J].

Ban, Yusuke ;

Honda, Chikako ;

Hatsuyama, Yoshimichi ;

Igarashi, Megumi ;

Bessho, Hideo ;

Moriguchi, Takaya .

PLANT AND CELL PHYSIOLOGY, 2007, 48 (07) :958-970

[3] An efficient method for rooting and acclimation of micropropagated apple cultivars [J].

Bolar, JP ;

Norelli, JL ;

Aldwinckle, HS ;

Hanke, V .

HORTSCIENCE, 1998, 33 (07) :1251-1252

[4] Mapping a candidate gene (MdMYB10) for red flesh and foliage colour in apple [J].

Chagne, David ;

Carlisle, Charmaine M. ;

Blond, Celine ;

Volz, Richard K. ;

Whitworth, Claire J. ;

Oraguzie, Nnadozie C. ;

Crowhurst, Ross N. ;

Allan, Andrew C. ;

Espley, Richard V. ;

Hellens, Roger P. ;

Gardiner, Susan E. .

BMC GENOMICS, 2007, 8 (1) :212

[5] Identification of co dominant RAPD markers tightly linked to fruit skin color in apple [J].

Cheng, FS ;

Weeden, NF ;

Brown, SK .

THEORETICAL AND APPLIED GENETICS, 1996, 93 (1-2) :222-227

[6] ENGINEERING HERBICIDE RESISTANCE IN PLANTS BY EXPRESSION OF A DETOXIFYING ENZYME [J].

DEBLOCK, M ;

BOTTERMAN, J ;

VANDEWIELE, M ;

DOCKX, J ;

THOEN, C ;

GOSSELE, V ;

MOVVA, NR ;

THOMPSON, C ;

VANMONTAGU, M ;

LEEMANS, J .

EMBO JOURNAL, 1987, 6 (09) :2513-2518

[7]

Doyle J.J., 1987, Focus, V19, P11, DOI DOI 10.2307/2419362

[8] Red colouration in apple fruit is due to the activity of the MYB transcription factor, MdMYB10 [J].

Espley, Richard V. ;

Hellens, Roger P. ;

Putterill, Jo ;

Stevenson, David E. ;

Kutty-Amma, Sumathi ;

Allan, Andrew C. .

PLANT JOURNAL, 2007, 49 (03) :414-427

[9] Development of an STS map of an interspecific progeny of Malus [J].

Fernandez-Fernandez, F. ;

Evans, K. M. ;

Clarke, J. B. ;

Govan, C. L. ;

James, C. M. ;

Maric, S. ;

Tobutt, K. R. .

TREE GENETICS & GENOMES, 2008, 4 (03) :469-479

[10] Redirection of flavonoid biosynthesis through the down-regulation of an anthocyanidin glucosyltransferase in ripening strawberry fruit [J].

Griesser, Markus ;

Hoffmann, Thomas ;

Bellido, Mari Luz ;

Rosati, Carlo ;

Fink, Barbara ;

Kurtzer, Robert ;

Aharoni, Asaph ;

Munoz-Blanco, Juan ;

Schwab, Wilfried .

PLANT PHYSIOLOGY, 2008, 146 (04) :1528-1539

← 1 2 3 4 5 →