Anti-Inflammatory Effect of Buckwheat Sprouts in Lipopolysaccharide-Activated Human Colon Cancer Cells and Mice

被引：34

作者：

Ishii, Satoshi ^{[1
]}

Katsumura, Takafumi ^{[1
]}

Shiozuka, Chikara ^{[1
]}

Ooyauchi, Keisuke ^{[1
]}

Kawasaki, Kunito ^{[1
]}

Takigawa, Shigenobu ^{[2
]}

Fukushima, Tatsunobu ^{[3
]}

Tokuji, Yoshihiko ^{[1
]}

Kinoshita, Mikio ^{[1
]}

Ohnishi, Masao ^{[1
]}

Kawahara, Mika ^{[4
]}

Ohba, Kiyoshi ^{[4
]}

机构：

[1] Obihiro Univ Agr & Vet Med, Dept Agr & Life Sci, Obihiro, Hokkaido 0808555, Japan

[2] Natl Agr Res Ctr Hokkaido Reg, Memuro, Hokkaido 0820071, Japan

[3] Mitsubishi Rayon Co Ltd, Yokohama Res Labs, Kanagawa 2300053, Japan

[4] Hokkaido Tokachi Area Reg Food Proc Technol Ctr, Obihiro, Hokkaido 0802462, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2008年 / 72卷 / 12期

关键词：

anti-inflammatory effect; buckwheat sprouts; lipopolysaccharide-activated cells; cytokine expression;

D O I：

10.1271/bbb.80324

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In conducting an in vitro screening of ethanol extracts from various natural foods using a human colon cancer cell line (CoLoTC cells), an extract of buckwheat sprouts (ExtBS) was found to express significant anti-inflammatory activity. The anti-inflammatory activity of ExtBS was confirmed by oral administration of lipopolysaccharide (LPS) to mice. Inflammatory cytokines (interleukin 6 and tumor necrosis factor alpha) were markedly up-regulated in the spleen and liver from LPS-administrated mice, and combinatory treatment with LPS and ExtBS decreased up-regulation of them in both cytokines. Both serum cytokine levels corresponded to their gene expressions in tissues, but no anti-inflammatry effect in mice was observed when ExtBS was treated intra peritoneally. ExtBS oral administration also showed protective activity as to hepatic injury induced by galactosamine/LPS treatment. Based on these data, we suggest that ExtBS contains anti-inflammatory compounds.

引用

页码：3148 / 3157

页数：10

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