A Novel Bacterium-Free Method for Generation of Flavivirus Infectious DNA by Circular Polymerase Extension Reaction Allows Accurate Recapitulation of Viral Heterogeneity

被引：72

作者：

Edmonds, Judith ^{[1
]}

van Grinsven, Erinke ^{[1
,2
]}

Prow, Natalie ^{[1
]}

Bosco-Lauth, Angela ^{[3
]}

Brault, Aaron C. ^{[3
]}

Bowen, Richard A. ^{[4
]}

Hall, Roy A. ^{[1
]}

Khromykh, Alexander A. ^{[1
]}

机构：

[1] Univ Queensland, Sch Chem & Mol Biosci, Australian Infect Dis Res Ctr, Brisbane, Qld, Australia

[2] Univ Utrecht, Grad Sch Life Sci, Utrecht, Netherlands

[3] Ctr Dis Control & Prevent, Div Vector Borne Dis, Ft Collins, CO USA

[4] Colorado State Univ, Ft Collins, CO 80523 USA

来源：

JOURNAL OF VIROLOGY | 2013年 / 87卷 / 04期

基金：

英国医学研究理事会;

关键词：

WEST-NILE-VIRUS; JAPANESE-ENCEPHALITIS-VIRUS; FULL-LENGTH CDNA; ENVELOPE PROTEIN; KUNJIN VIRUS; IN-VITRO; RT-PCR; RNA; STRAIN; CLONE;

D O I：

10.1128/JVI.03162-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A novel bacterium-free approach for rapid assembly of flavivirus infectious cDNAs using circular polymerase extension reaction was applied to generate infectious cDNA for the virulent New South Wales isolate of the Kunjin strain of West Nile virus (KUNV) that recently emerged in Australia. Recovered virus recapitulated the genetic heterogeneity present in the original isolate. The approach was utilized to generate viral mutants with designed phenotypic properties and to identify E protein glycosylation as one of the virulence determinants.

引用

页码：2367 / 2372

页数：6

共 26 条

[1] Virulence determinants between New York 99 and Kunjin strains of West Nile virus [J].

Audsley, Michelle ;

Edmonds, Judith ;

Liu, Wenjun ;

Mokhonov, Vlad ;

Mokhonova, Ekaterina ;

Melian, Ezequeil Balmori ;

Prow, Natalie ;

Hall, Roy A. ;

Khromykh, Alexander A. .

VIROLOGY, 2011, 414 (01) :63-73

[2] Envelope protein glycosylation status influences mouse neuroinvasion phenotype of genetic lineage 1 West Nile Virus strains [J].

Beasley, DWC ;

Whiteman, MC ;

Zhang, SL ;

Huang, CYH ;

Schneider, BS ;

Smith, DR ;

Gromowski, GD ;

Higgs, S ;

Kinney, RM ;

Barrett, ADT .

JOURNAL OF VIROLOGY, 2005, 79 (13) :8339-8347

[3] A stable full-length yellow fever virus cDNA clone and the role of conserved RNA elements in flavivirus replication [J].

Bredenbeek, PJ ;

Kooi, EA ;

Lindenbach, B ;

Huijkman, N ;

Rice, CM ;

Spaan, WJM .

JOURNAL OF GENERAL VIROLOGY, 2003, 84 :1261-1268

[4] Characterization of Virulent West Nile Virus Kunjin Strain, Australia, 2011 [J].

Frost, Melinda J. ;

Zhang, Jing ;

Edmonds, Judith H. ;

Prow, Natalie A. ;

Gu, Xingnian ;

Davis, Rodney ;

Hornitzky, Christine ;

Arzey, Kathleen E. ;

Finlaison, Deborah ;

Hick, Paul ;

Read, Andrew ;

Hobson-Peters, Jody ;

May, Fiona J. ;

Doggett, Stephen L. ;

Haniotis, John ;

Russell, Richard C. ;

Hall, Roy A. ;

Khromykh, Alexander A. ;

Kirkland, Peter D. .

EMERGING INFECTIOUS DISEASES, 2012, 18 (05) :792-800

[5] Infectious transcripts of tick-borne encephalitis virus, generated in days by RT-PCR [J].

Gritsun, TS ;

Gould, EA .

VIROLOGY, 1995, 214 (02) :611-618

[6] DNA vaccine coding for the full-length infectious Kunjin virus RNA protects mice against the New York strain of West Nile virus [J].

Hall, RA ;

Nisbet, DJ ;

Pham, KB ;

Pyke, AT ;

Smith, GA ;

Khomykh, AA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (18) :10460-10464

[7] EPITOPE ANALYSIS OF THE ENVELOPE AND NONSTRUCTURAL GLYCOPROTEINS OF MURRAY VALLEY ENCEPHALITIS-VIRUS [J].

HALL, RA ;

KAY, BH ;

BURGESS, GW ;

CLANCY, P ;

FANNING, ID .

JOURNAL OF GENERAL VIROLOGY, 1990, 71 :2923-2930

[8] MONOCLONAL-ANTIBODIES TO KUNJIN AND KOKOBERA VIRUSES [J].

HALL, RA ;

BURGESS, GW ;

KAY, BH ;

CLANCY, P .

IMMUNOLOGY AND CELL BIOLOGY, 1991, 69 :47-49

[9] Monoclonal antibodies to the West Nile virus NS5 protein map to linear and conformational epitopes in the methyltransferase and polymerase domains [J].

Hall, Roy A. ;

Tan, Si En ;

Selisko, Barbara ;

Slade, Rachael ;

Hobson-Peters, Jody ;

Canard, Bruno ;

Hughes, Megan ;

Leung, Jason Y. ;

Balmori-Melian, Ezequiel ;

Hall-Mendelin, Sonja ;

Pham, Kim B. ;

Clark, David C. ;

Prow, Natalie A. ;

Khromykh, Alexander A. .

JOURNAL OF GENERAL VIROLOGY, 2009, 90 :2912-2922

[10] A glycosylated peptide in the West Nile virus envelope protein is immunogenic during equine infection [J].

Hobson-Peters, Jody ;

Toye, Philip ;

Sanchez, Melissa D. ;

Bossart, Katharine N. ;

Wang, Lin-Fa ;

Clark, David C. ;

Cheah, Wai Yuen ;

Hall, Roy A. .

JOURNAL OF GENERAL VIROLOGY, 2008, 89 :3063-3072

← 1 2 3 →