SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors

被引：62

作者：

Koike, Seiichi ^{[1
]}

Jahn, Reinhard ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept Neurobiol, D-37077 Gottingen, Germany

来源：

NATURE COMMUNICATIONS | 2019年 / 10卷 / 1期

关键词：

SYNDROME-ASSOCIATED PROTEIN; RAB5 EFFECTOR EEA1; MEMBRANE-FUSION; HOMOTYPIC FUSION; COMPLEX; TRANSPORT; YEAST; ENDOSOMES; REVEALS; LINK;

D O I：

10.1038/s41467-019-09617-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Membrane traffic operates by vesicles that bud from precursor organelles and are transported to their target compartment where they dock and fuse. Targeting requires tethering factors recruited by small GTPases and phosphoinositides whereas fusion is carried out by SNARE proteins. Here we report that vesicles containing the Q-SNAREs syntaxin 13 (Stx13) and syntaxin 6 (Stx6) together are targeted to a different endosomal compartment than vesicles containing only Stx6 using injection of artificial vesicles. Targeting by Stx6 requires Vps51, a component of the GARP/EARP tethering complexes. In contrast, targeting by both Stx6 and Stx13 is governed by Vps13B identified here as tethering factor functioning in transport from early endosomes to recycling endosomes. Vps13B specifically binds to Stx13/Stx6 as well as to Rab14, Rab6, and PtdIns(3)P. We conclude that SNAREs use a combinatorial code for recruiting tethering factors, revealing a key function in targeting that is independent of SNARE pairing during fusion.

引用

页数：16

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