Universal fractionation of lignin-carbohydrate complexes (LCCs) from lignocellulosic biomass: an example using spruce wood

被引:110
作者
Du, Xueyu [1 ]
Gellerstedt, Goran [1 ]
Li, Jiebing [1 ]
机构
[1] Royal Inst Technol, Kungliga Tekn Hgsk, Dept Fibre & Polymer Technol, SE-10044 Stockholm, Sweden
基金
美国国家卫生研究院; 美国国家科学基金会; 美国农业部;
关键词
lignin-carbohydrate complex (LCC); universal fractionation approach; spruce; NMR; thioacidolysis; pyrolysis-GC; SOFTWOOD KRAFT PULP; BONDS; BIRCH; ABIES;
D O I
10.1111/tpj.12124
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
It is of both theoretical and practical importance to develop a universally applicable approach for the fractionation and sensitive lignin characterization of lignin-carbohydrate complexes (LCCs) from all types of lignocellulosic biomass, both natively and after various types of processing. In the present study, a previously reported fractionation approach that is applicable for eucalyptus (hardwood) and flax (non-wood) was further improved by introducing an additional step of barium hydroxide precipitation to isolate the mannan-enriched LCC (glucomannan-lignin, GML), in order to suit softwood species as well. Spruce wood was used as the softwood sample. As indicated by the recovery yield and composition analysis, all of the lignin was recovered in three LCC fractions: a glucan-enriched fraction (glucan-lignin, GL), a mannan-enriched fraction (GML) and a xylan-enriched fraction (xylan-lignin, XL). All of the LCCs had high molecular masses and were insoluble or barely soluble in a dioxane/water solution. Carbohydrate and lignin signals were observed in H-1 NMR, C-13 CP-MAS NMR and normal- or high-sensitivity 2D HSQC NMR analyses. The carbohydrate and lignin constituents in each LCC fraction are therefore believed to be chemically bonded rather than physically mixed with one another. The three LCC fractions were found to be distinctly different from each other in terms of their lignin structures, as revealed by highly sensitive analyses by thioacidolysis-GC, thioacidolysis-SEC and pyrolysis-GC.
引用
收藏
页码:328 / 338
页数:11
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