Real-time PCRs assay for serogrouping Listeria monocytogenes and differentiation from other Listeria spp.

被引：29

作者：

Vitullo, Monia ^{[1
,2
]}

Grant, Kathie Ann ^{[1
]}

Sammarco, Michela Lucia ^{[2
]}

Tamburro, Manuela ^{[2
]}

Ripabelli, Giancarlo ^{[2
]}

Amar, Corinne Francoise Laurence ^{[1
]}

机构：

[1] Hlth Protect Agcy, Lab Gastrointestinal Pathogens, London NW9 5EQ, England

[2] Univ Molise, Dept Med & Hlth Sci, Campobasso, Italy

来源：

MOLECULAR AND CELLULAR PROBES | 2013年 / 27卷 / 01期

关键词：

Listeria spp; Listeria monocytogenes; Serogrouping; Real-time PCR; Molecular epidemiology;

D O I：

10.1016/j.mcp.2012.10.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A 5'-exonuclease real-time triplex-PCR assay was developed for serogrouping Listeria monocytogenes, and differentiation from other Listeria spp. The assay was evaluated on 109 Listeria cultures, and results were compared with a previously validated gel-based multiplex-PCR procedure. All L. monocytogenes were correctly classified into four serogroups, including atypical serotype 4b strains, and differentiated from other Listeria species. The assay is a rapid method for categorisation of suspect L monocytogenes. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：68 / 70

页数：3

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