Detection of Quorum Sensing Signal Molecules in Edwardsiella ictaluri Ei-151

被引:3
作者
Yang, Qian [1 ,2 ,3 ]
Han, Yin [1 ]
Nguyen Thi Ngoc Tinh [4 ]
Nguyen Thi Hien [4 ]
Bossier, Peter [2 ,3 ]
机构
[1] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
[2] Univ Ghent, Lab Aquaculture, B-9000 Ghent, Belgium
[3] Univ Ghent, Artemia Reference Ctr, B-9000 Ghent, Belgium
[4] Res Inst Aquaculture 2, Ho Chi Minh City, Vietnam
基金
中国国家自然科学基金;
关键词
Edwardsiella ictaluri; Quorum sensing; N-acylhomoserine lactones (AHLs); Autoinducer-2 (AI-2); Cholerae autoinducer-1 (CAI-1); N-ACYLHOMOSERINE LACTONE; VIBRIO-HARVEYI; TARDA STRAIN; BACTERIA; AUTOINDUCER-2; GENE; LUXS; EXPRESSION; INDUCTION; VIRULENCE;
D O I
10.1007/s12088-012-0312-9
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Edwardsiella ictaluri is a Gram-negative pathogenic bacterium in the family Enterobacteriaceae that causes enteric septicemia of catfish, which has become a significant problem in the aquaculture of striped catfish (Pangasianodon hypophthalmus) in Vietnam. In this study, a bacterium designated as Ei-151 was isolated from diseased striped catfish and proved to be virulent. Based on 16S rDNA sequencing and phenotypic tests, the pathogenic bacterium was identified as Edw. ictaluri. The presence of quorum sensing signal molecules in Edw. ictaluri Ei-151 was detected with different biosensor strains. The results showed that Ei-151 produced at least three kinds of acylated homoserine lactone (AHL) signal molecules as detected with the biosensor Agrobacterium tumefaciens KYC55, and the AHLs fingerprint was similar to that of Edw. tarda. During its entire growth, the levels of AHLs and autoinducer-2 produced by Ei-151 peaked at the stationary phase (OD600 1.8), which suggested that both of them may function at the stationary phase. No Cholerae autoinducer-1-like activity (including Edw. ictaluri LMG7860(T)) was detected.
引用
收藏
页码:581 / 586
页数:6
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