Anti-CD3 priming generates heterogeneous antigen-specific memory CD4 T cells

被引:12
作者
Patke, DS [1 ]
Ahmadzadeh, M [1 ]
Bingaman, AW [1 ]
Farber, DL [1 ]
机构
[1] Univ Maryland, Sch Med, Dept Surg, Div Transplantat, Baltimore, MD 21201 USA
关键词
T lymphocytes; memory; T cell receptors; cellular differentiation; cellular activation; immunotherapy; influenza; primary response;
D O I
10.1016/j.clim.2005.07.012
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Anti-CD3 activation of peripheral T cells is used in adoptive immunotherapy for cancer and HIV infection, but the long-term fate of anti-CD3-primed T cells in vivo is not known. In this study, we demonstrate that anti-CD3-mediated activation of influenza hemagglutinin (HA)-specific TCR-transgenic CD4 T cells results in generation of a long-lived HA-specific memory CD4 T cell population when transferred into lymphocyte-deficient and intact mouse hosts. This anti-CD3-primed memory population is indistinguishable from HA peptide-primed memory CD4 T cells in terms of phenotype, rapid recall function, and enhanced proliferative capacity. Moreover, anti-CD3 priming generates phenotypically heterogeneous memory subsets in lymphoid and non-lymphoid sites. Our results suggest that anti-CD3 has potential efficacy in generating memory responses in adoptive immunotherapies and vaccines and that the tissue distribution and maintenance of heterogeneous lymphoid and non-lymphoid memory T cell subsets are a stochastic process that can occur independent of antigen or TCR specificity. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码:125 / 132
页数:8
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