The blocking of aquaporin-3 (AQP3) impairs extravillous trophoblast cell migration

被引：28

作者：

Alejandra, Reca ^{[1
]}

Natalia, Szpilbarg ^{[1
]}

Damiano Alicia, E. ^{[1
,2
]}

机构：

[1] Univ Buenos Aires, Fac Med, CONICET, Lab Biol Reprod,Inst Fisiol & Biofis Bernardo Hou, Buenos Aires, DF, Argentina

[2] Univ Buenos Aires, Fac Farm & Bioquim, Dept Ciencias Biol, Catedra Biol Celular & Mol, Junin 956 1er Piso, RA-1113 Buenos Aires, DF, Argentina

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2018年 / 499卷 / 02期

关键词：

Trophoblast; Aquaporin-3; Placentation; Migration; Preeclampsia; WATER CHANNEL; HUMAN-PREGNANCY; EXPRESSION; PREECLAMPSIA; PLACENTAS;

D O I：

10.1016/j.bbrc.2018.03.133

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several aquaporins (AQPs) are expressed in extravillous (EVT) and villous trophoblast cells. Among them, AQP3 is the most abundant AQP expressed in chorionic villi samples from first trimester, followed by AQP1 and AQP9. Although AQP3 expression persists in term placentas, it is significantly decreased in placentas from preeclamptic pregnancies. AQP3 is involved in the migration of different cell types, however its role in human placenta is still unknown. Here, we evaluated the role of AQP3 in the migration of EVT cells during early gestation. Our results showed that Swan 71 cells expressed AQP1, AQP3 and AQP9 but only the blocking of AQP3 by CuSO4 or the silencing of its expression by siRNA significantly attenuates EVT cell migration. Our work provides evidence that AQP3 is required for EVT cell migration and suggests that an altered expression of placental AQP3 may produce failures in placentation such as in preeclampsia. (C) 2018 Elsevier Inc. All rights reserved.

引用

页码：227 / 232

页数：6

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