Enhanced Production of Laccase from Coriolus versicolor NCIM 996 by Nutrient Optimization Using Response Surface Methodology

被引：24

作者：

Arockiasamy, Santhiagu ^{[1
]}

Krishnan, Indira Packialakshmi Gurusamy ^{[1
]}

Anandakrishnan, Nimalanandan ^{[1
]}

Seenivasan, Sabitha ^{[1
]}

Sambath, Agalya ^{[1
]}

Venkatasubramani, Janani Priya ^{[1
]}

机构：

[1] Kalasalingam Univ, Dept Biotechnol, Krishnankoil 626190, Tamil Nadu, India

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2008年 / 151卷 / 2-3期

关键词：

Laccase; Plackett and Burman design; Central composite design; Enzyme activity; Coriolus versicolor;

D O I：

10.1007/s12010-008-8205-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plackett and Burman design criterion and central composite design were applied successfully for enhanced production of laccase by Coriolus versicolor NCIM 996 for the first time. Plackett and Burman design criterion was applied to screen the significance of ten nutrients on laccase production by C. versicolor NCIM 996. Out of the ten nutrients tested, starch, yeast extract, MnSO4, MgSO4 center dot 7H(2)O, and phenol were found to have significant effect on laccase production. A central composite design was applied to determine the optimum concentrations of the significant variables obtained from Plackett-Burman design. The optimized medium composition for production of laccase was (g/l): starch, 30.0; yeast extract, 4.53; MnSO4, 0.002; MgSO4 center dot 7H(2)O, 0.755; and phenol, 0.026, and the optimum laccase production was 6,590.26 (U/l), which was 7.6 times greater than the control.

引用

页码：371 / 379

页数：9

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