MdVHA-A encodes an apple subunit A of vacuolar H+-ATPase and enhances drought tolerance in transgenic tobacco seedlings

被引：29

作者：

Dong, Qing-Long ^{[1
,2
]}

Wang, Chun-Rong ^{[1
]}

Liu, Dan-Dan ^{[1
]}

Hu, Da-Gang ^{[1
]}

Fang, Mou-Jing ^{[1
]}

You, Chun-Xiang ^{[1
]}

Yao, Yu-Xin ^{[1
]}

Hao, Yu-Jin ^{[1
]}

机构：

[1] Shandong Agr Univ, Natl Res Ctr Apple Engn & Technol, Coll Hort Sci & Engn, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China

[2] Shandong Inst Pomol, Tai An 271000, Shandong, Peoples R China

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2013年 / 170卷 / 06期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

Apple (Malus domestica B.); Vacuolar H+-ATPase gene; Gene expression; Lateral root; Drought tolerance; SALT STRESS; DIFFERENTIAL EXPRESSION; HYDROLYTIC ACTIVITY; OSMOTIC-STRESS; FREE PROLINE; RED BEET; V-ATPASE; TONOPLAST; GENES; PYROPHOSPHATASE;

D O I：

10.1016/j.jplph.2012.12.014

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Vacuole H+-ATPases (VHAs) are plant proton pumps, which play a crucial role in plant growth and stress tolerance. In the present study, we demonstrated that the apple vacuolar H+-ATPase subunit A (MdVHA-A) is highly conserved with subunit A of VHA (VHA-A) proteins from other plant species. MdVHA-A was expressed in vegetative and reproductive organs. In apple in vitro shoot cultures, expression was induced by polyethylene glycol (PEG)-mediated osmotic stress. We further verified that over-expression of MdVHA-A conferred transgenic tobacco seedlings with enhanced vacuole H+-ATPase (VHA) activity and improved drought tolerance. The enhanced PEG-mimic drought response of transgenic tobacco seedlings was related to an extended lateral root system (dependent on auxin translocation) and more efficient osmotic adjustment. Our results indicate that MdVHA-A is a candidate gene for improving drought tolerance in plants. (C) 2013 Elsevier GmbH. All rights reserved.

引用

页码：601 / 609

页数：9

共 56 条

[1] Early genes and auxin action [J].