Effect of beta 2glycoprotein I and human monoclonal anticardiolipin antibody on the protein S/C4b-binding protein system

被引：12

作者：

Atsumi, T

Khamashta, MA

Ames, PRJ

Ichikawa, K

Koike, T

Hughes, GRV

机构：

[1] ST THOMAS HOSP,RAYNE INST,LUPUS RES UNIT,LONDON SE1 7EH,ENGLAND

[2] HOKKAIDO UNIV,SCH MED,DEPT MED 2,SAPPORO,HOKKAIDO 060,JAPAN

来源：

LUPUS | 1997年 / 6卷 / 04期

关键词：

antiphospholipid antibody; antiphosolipid syndrome; coagulation; thrombosis;

D O I：

10.1177/096120339700600403

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The effect of beta 2glycoprotein I (beta 2GPI) and human monoclonal anticardiolipin antibody (aCL) on the protein S/C4b-binding protein (C4BP) system was evaluated. The binding of C4BP to protein S was assessed by ELISA in the presence of beta 2GPI with/without human monoclonal aCL. beta 2GPI downregulated the binding between S and C4BP significantly. Human monoclonal aCL abolished the beta 2GPI inhibitory effect in a calcium (Ca++) independent fashion. In separate experiments, the reactivity of aCL towards protein S in the presence or absence of beta 2GPI and cardiolipin was investigated. Monoclonal aCL bound to protein S only in the presence of a combination of beta 2GPI and cardiolipin. This binding was Ca++ dependent. These findings suggest that human monoclonal aCL increases the affinity of C4BP for protein S, and that protein S may represent one of the targets for aCL when combined with beta 2GPI and cardiolipin. Both issues may explain acquired free protein S deficiency and the attendant risk of thrombosis in patients with aCL.

引用

页码：358 / 364

页数：7

共 42 条

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