A high-resolution polymerase chain reaction-sequence-specific primer HLA-B*27 typing set and its application in routine HLA-B27 testing

被引：13

作者：

Downing, J. ^{[1
]}

Coates, E. ^{[1
]}

Street, J. ^{[1
]}

Hammond, L. ^{[1
]}

Rees, T. J. ^{[1
]}

Pepperall, J. ^{[1
]}

Darke, C. ^{[1
]}

机构：

[1] Welsh Blood Serv, Welsh Transplantat & Immogenet Lab, Pontyclun CF72 9WB, Wales

来源：

GENETIC TESTING | 2006年 / 10卷 / 02期

关键词：

D O I：

10.1089/gte.2006.10.98

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We designed a set of 35 polymerase chain reaction sequence- specific primers ( PCR- SSP) in 29 SSP mixtures to assign 29 HLA- B* 27 4- digit level alleles ( B* 2701- B* 2721 and B* 2723- B* 2730). This was used in conjunction with our 41 PCR- SSP primer mixture low- resolution HLA- B typing set to fully differentiate B* 27 from all other HLA- B alleles. Successful typing set validation used 521 B* 27 samples covering 13 ( B* 2701- B* 2710 and B* 2712, B* 2717, B* 2723) alleles. The distribution of B* 27 alleles was determined in a random population of 4020 local blood donors and the use of PCR- SSP B* 27 typing in our routine flow cytometry- based HLA- B27/ B2708 typing strategy is described.

引用

页码：98 / 103

页数：6

共 28 条

[1] HLA-B27 polymorphism [J].

Ball, EJ ;

Khan, MA .

JOINT BONE SPINE, 2001, 68 (05) :378-382

[2]

BREWERTON DA, 1973, LANCET, V1, P904

[3] MONOCLONAL ANTIBODIES FOR ANALYSIS OF THE HLA SYSTEM [J].

BRODSKY, FM ;

PARHAM, P ;

BARNSTABLE, CJ ;

CRUMPTON, MJ ;

BODMER, WF .

IMMUNOLOGICAL REVIEWS, 1979, 47 :3-61

[4] HLA class I associations of ankylosing spondylitis in the white population in the united Kingdom [J].

Brown, MA ;

Pile, KD ;

Kennedy, LG ;

Calin, A ;

Darke, C ;

Bell, J ;

Wordsworth, BP ;

Cornelis, F .

ANNALS OF THE RHEUMATIC DISEASES, 1996, 55 (04) :268-270

[5] Routine HLA-B27 typing by flow cytometry: differentiation of the products of HLA-B*2702, B*2705 and B*2708 [J].

Coates, E ;

Darke, C .

EUROPEAN JOURNAL OF IMMUNOGENETICS, 1998, 25 (01) :29-37

[6]

DAMATO M, 1995, DIS MARKERS, V12, P215

[7] An overview of the Welsh Bone Marrow Donor Registry: 10 years of bone marrow donor provision [J].

Darke, C .

BONE MARROW TRANSPLANTATION, 2000, 25 (07) :771-777

[8] Immunogenetic study of a new HLA allele, B*2723 [J].

Darke, C ;

Street, J ;

Hammond, L ;

Downing, J ;

Thompson, J .

TISSUE ANTIGENS, 2002, 60 (05) :400-403

[9] Five-locus HLA typing of hematopoietic stem cell donor volunteers using PCR sequence specific primers [J].

Downing, J ;

Guttridge, MG ;

Thompson, J ;

Darke, C .

GENETIC TESTING, 2004, 8 (03) :301-312

[10] SEROLOGICAL IDENTIFICATION OF A NEW HLA-B7 VARIANT ANTIGEN - HLA-B7QUI [J].

FUSSELL, H ;

THOMAS, M ;

STREET, J ;

DARKE, C .

EUROPEAN JOURNAL OF IMMUNOGENETICS, 1994, 21 (01) :23-31

← 1 2 3 →