Therapeutic Doses of Nonsteroidal Anti-Inflammatory Drugs Inhibit Osteosarcoma MG-63 Osteoblast-Like Cells Maturation, Viability, and Biomineralization Potential

被引:31
作者
De Luna-Bertos, E. [1 ]
Ramos-Torrecillas, J. [1 ]
Garcia-Martinez, O. [1 ]
Guildford, A. [2 ]
Santin, M. [2 ]
Ruiz, C. [1 ,3 ]
机构
[1] Univ Granada, Fac Hlth Sci, Dept Nursing, E-18071 Granada, Spain
[2] Univ Brighton, Sch Pharm & Biomol Sci, Brighton BN2 4G1, E Sussex, England
[3] Univ Granada, Fac Med, Inst Neurosci, Granada 18100, Spain
关键词
ANTIGENIC PHENOTYPE; CYCLOOXYGENASE-2; INHIBITORS; PHAGOCYTIC CAPACITY; IFN-GAMMA; DIFFERENTIATION; PROLIFERATION; EXPRESSION; IBUPROFEN; ADHESION; GROWTH;
D O I
10.1155/2013/809891
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Nonsteroidal anti-inflammatory drugs (NSAIDs) are frequently used to reduce pain and inflammation. However, their effect on bone metabolisms is not well known, and results in the literature are contradictory. The present study focusses on the effect of dexketoprofen, ketorolac, metamizole, and acetylsalicylic acid, at therapeutic doses, on different biochemical and phenotypic pathways in human osteoblast-like cells. Osteoblasts (MG-63 cell line) were incubated in culture medium with 1-10 mu M of dexketoprofen, ketorolac, metamizole, and acetylsalicylic acid. Flow cytometry was used to study antigenic profile and phagocytic activity. The osteoblastic differentiation was evaluated by mineralization and synthesis of collagen fibers by microscopy and alkaline phosphatase activity (ALP) by spectrophotometric assay. Short-term treatment with therapeutic doses of NSAIDs modulated differentiation, antigenic profile, and phagocyte activity of osteoblast-like cells. The treatment reduced ALP synthesis and matrix mineralization. However, nonsignificant differences were observed on collagen syntheses after treatments. The percentage of CD54 expression was increased with all treatments. CD80, CD86, and HLA-DR showed a decreased expression, which depended on NSAID and the dose applied. The treatments also decreased phagocyte activity in this cellular population. The results of this paper provide evidences that NSAIDs inhibit the osteoblast differentiation process thus reducing their ability to produce new bone mineralized extracellular matrix.
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页数:13
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