Interpenetrating networks based on gelatin methacrylamide and PEG formed using concurrent thiol click chemistries for hydrogel tissue engineering scaffolds

被引:197
作者
Daniele, Michael A. [1 ]
Adams, Andre A. [2 ]
Naciri, Jawad [2 ]
North, Stella H. [2 ]
Ligler, Frances S. [2 ]
机构
[1] Natl Acad Sci, Natl Res Council, Ctr Bio Mol Sci & Engn, Naval Res Lab, Washington, DC 20375 USA
[2] Ctr Bio Mol Sci & Engn, Naval Res Lab, Washington, DC 20375 USA
关键词
Interpenetrating network (IPN); Extracellular matrix; Thiol-yne; Click chemistry; Gelatin; Poly(ethylene glycol); PHOTO-CROSS-LINKING; POLY(ETHYLENE GLYCOL); PHOTOCROSSLINKABLE GELATIN; STATISTICAL-MECHANICS; CELL; CYTOCOMPATIBILITY; POLYMERIZATION; IMMOBILIZATION; PROLIFERATION; ENTRAPMENT;
D O I
10.1016/j.biomaterials.2013.11.009
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The integration of biological extracellular matrix (ECM) components and synthetic materials is a promising pathway to fabricate the next generation of hydrogel-based tissue scaffolds that more accurately emulate the microscale heterogeneity of natural ECM. We report the development of a bio/synthetic interpenetrating network (BioSIN(x)), containing gelatin methacrylamide (GelMA) polymerized within a poly(ethylene glycol) (PEG) framework to form a mechanically robust network capable of supporting both internal cell encapsulation and surface cell adherence. The covalently crosslinked PEG network was formed by thiol-yne coupling, while the bioactive GelMA was integrated using a concurrent thiol-ene coupling reaction. The physical properties (i.e. swelling, modulus) of BioSINx were compared to both PEG networks with physically-incorporated gelatin (BioSIN(p)) and homogenous hydrogels. BioSIN(x) displayed superior physical properties and significantly lower gelatin dissolution. These benefits led to enhanced cytocompatibility for both cell adhesion and encapsulation; furthermore, the increased physical strength provided for the generation of a micro-engineered tissue scaffold. Endothelial cells showed extensive cytoplasmic spreading and the formation of cellular adhesion sites when cultured onto BioSINx; moreover, both encapsulated and adherent cells showed sustained viability and proliferation. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1845 / 1856
页数:12
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