Multi-resolution open-top light-sheet microscopy to enable efficient 3D pathology workflows

被引：26
作者：
Barner, Lindsey A. [1 ]
Glaser, Adam K. [1 ]
Huang, Hongyi [2 ]
True, Lawrence D. [2 ]
Liu, Jonathan T. C. [1 ,2 ,3 ]
机构：
[1] Univ Washington, Dept Mech Engn, Seattle, WA 98195 USA
[2] Univ Washington, Dept Lab Med & Pathol, Seattle, WA 98195 USA
[3] Univ Washington, Dept Bioengn, Seattle, WA 98195 USA
来源：
BIOMEDICAL OPTICS EXPRESS | 2020年 / 11卷 / 11期
基金：
美国国家科学基金会;
关键词：
PLANE ILLUMINATION MICROSCOPY; FIELD; EMBRYOS; GUIDE;
D O I：
10.1364/BOE.408684
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Open-top light-sheet (OTLS) microscopes have been developed for user-friendly and versatile high-throughput 3D microscopy of thick specimens. As with all imaging modalities, spatial resolution trades off with imaging and analysis times. A hierarchical multi-scale imaging workflow would therefore be of value for many volumetric microscopy applications. We describe a compact multi-resolution OTLS microscope, enabled by a novel solid immersion meniscus lens (SIMlens), which allows users to rapidly transition between air-based objectives for low- and high-resolution 3D imaging. We demonstrate the utility of this system by showcasing an efficient 3D analysis workflow for a diagnostic pathology application. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
引用
收藏
页码：6605 / 6619
页数：15
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