Label-free DNA sensor for Pb2+ based on a duplex-quadruplex exchange

被引：15

作者：

Zhang, Lingli ^{[1
]}

Mi, Naxiu ^{[1
]}

Zhang, Youyu ^{[1
]}

Wei, Mingjie ^{[1
]}

Li, Haitao ^{[1
]}

Yao, Shouzhuo ^{[1
]}

机构：

[1] Hunan Normal Univ, Coll Chem & Chem Engn, Key Lab Chem Biol & Tradit Chinese Med Res, Minist Educ, Changsha 410081, Hunan, Peoples R China

来源：

ANALYTICAL METHODS | 2013年 / 5卷 / 21期

基金：

中国国家自然科学基金;

关键词：

ON FLUORESCENCE DETECTION; GOLD NANOPARTICLES; LEAD IONS; COLORIMETRIC DETECTION; HIGH SELECTIVITY; DNAZYME; BIOSENSOR; GRAPHENE; CADMIUM; SURFACE;

D O I：

10.1039/c3ay41032c

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple label-free Pb2+ fluorescent aptasensor was proposed in this work. It is known that SYBR Green1 can embed into double-stranded DNA resulting in fluorescence enhancement, but it cannot bind to the G-quadruplex formed by Pb2+ and PS2.M. In the absence of Pb2+, SYBR Green1 intercalated into the double-stranded DNA hybridized between PS2.M and its complementary strand and the fluorescence was greatly enhanced. In the presence of Pb2+, a compact G-quadruplex structure was formed and the double-stranded structure was destroyed, releasing SYBR Green1 and causing the fluorescence intensity to significantly decrease. The detection limit of the sensor was as low as 3 nM. This proposed assay strategy was a low-cost detection method for Pb2+ with high sensitivity and good selectivity.

引用

收藏

页码：6100 / 6105

页数：6

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