The effect of hyperbaric oxygen on nitric oxide synthase activity and expression in ischemia-reperfusion injury

被引:26
作者
Baynosa, Richard C. [1 ]
Naig, Anna L. [1 ]
Murphy, Patrick S. [1 ]
Fang, Xin Hua [1 ]
Stephenson, Linda L. [1 ]
Khiabani, Kayvan T. [1 ]
Wang, Wei Z. [1 ]
Zamboni, William A. [1 ]
机构
[1] Univ Nevada, Sch Med, Div Plast Surg, Dept Surg, Las Vegas, NV 89102 USA
关键词
Hyperbaric oxygen; Nitric oxide synthase; Nitric oxide; Ischemia-reperfusion; Nitric oxide synthase activity; CEREBRAL ISCHEMIA/REPERFUSION INJURY; NEUTROPHIL CD18 POLARIZATION; SKELETAL-MUSCLE; ENOS PHOSPHORYLATION; OXIDATIVE STRESS; GENE-EXPRESSION; NO-REFLOW; PROTECTION; THERAPY; PATHWAY;
D O I
10.1016/j.jss.2013.01.004
中图分类号
R61 [外科手术学];
学科分类号
摘要
Background: Hyperbaric oxygen (HBO) mitigates ischemia-reperfusion (IR) injury via a nitric oxide mechanism that is nitric oxide synthase (NOS) dependent. The purpose of this study was to investigate this NOS-dependent mechanism by examining isoform-specific, tissue-specific, and time-specific upregulation of NOS mRNA, protein, and enzymatic activity. Methods: We raised a gracilis flap in Wistar rats that were separated into early and late phases. Treatment groups included nonischemic control, IR, HBO-treated ischemia-reperfusion (IR-HBO), and nonischemic HBO control. We harvested tissue-specific samples from gracilis, rectus femoris, aorta, and pulmonary tissues and processed them by reverse transcription polymerase chain reaction and Western blot to determine upregulation of isoform-specific NOS mRNA and protein. We also harvested tissue for NOS activity to investigate upregulation of enzymatic activity. Data are presented as mean +/- standard error of the mean with statistics performed by analysis of variance. P <= 0.05 was considered significant. Results: There was no increase in NOS mRNA in the early phase. In the late phase, there was a significant increase in endothelial-derived NOS (eNOS) mRNA in IR-HBO compared with IR in gracilis muscle (79.4 +/- 22.3 versus 36.1 +/- 4.5; P < 0.05) and pulmonary tissues (91.0 +/- 31.2 versus 30.2 +/- 3.1; P < 0.01). There was a significant increase in the late-phase eNOS pulmonary protein IR-HBO group compared with IR (235.5 +/- 46.8 versus 125.2 +/- 14.7; P < 0.05). Early-phase NOS activity was significantly increased in IR-HBO compared with IR in pulmonary tissue only (0.049 +/- 0.009 versus 0.023 +/- 0.003; P < 0.05). Conclusions: The NOS-dependent effects of HBO on IR injury may result from a systemic effect involving an early increase in eNOS enzymatic activity followed by a late-phase increase in eNOS protein expression within the pulmonary tissues. (C) 2013 Elsevier Inc. All rights reserved.
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页码:355 / 361
页数:7
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