Distribution and Abundance of Antibiotic Resistance Genes in Sand Settling Reservoirs and Drinking Water Treatment Plants across the Yellow River, China

被引:44
作者
Lu, Junying [1 ,2 ]
Tian, Zhe [1 ,2 ]
Yu, Jianwei [1 ,2 ]
Yang, Min [1 ,2 ]
Zhang, Yu [1 ,2 ]
机构
[1] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Aquat Chem, Beijing 100085, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
基金
中国国家自然科学基金;
关键词
antibiotic resistance gene; sand settling reservoirs; drinking water treatment plants; the Yellow River; WASTE-WATER; PREVALENCE; DIVERSITY; BIOFILMS; BACTERIA; CONTAMINATION; COMMUNITY; ELEMENTS; SHIFT;
D O I
10.3390/w10030246
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Understanding how antibiotic resistance genes (ARGs) are distributed in drinking water treatment processes is important due to their potential public health risk. Little is known about the occurrence and distribution of ARGs in typical drinking water treatment processes, such as sand settling reservoirs (SSRs) and drinking water treatment plants (DWTPs), in the Yellow River, especially at the catchment scale. In this study, ARG profiling was investigated from water samples of influent (river water) and effluent (source water) of SSRs and finished water of DWTPs in six cities along the Yellow River catchment using real-time quantitative polymerase chain reaction (qPCR) and 16S rRNA gene sequencing. Seventeen ARGs and two mobile genetic elements (MGEs) were detected, among which aadE, strA, strB, tetA, sulII, intl1, and Tn916 had high detection rates (over 80%). The absolute abundances (gene copies/mL of water) of ARGs were reduced by the SSRs and DWTPs generally, but no reductions were observed for the relative abundances (gene copies/16S rRNA gene) of ARGs. Spatial distributions of ARGs and bacteria were not observed. The distribution of bacterial genera was clustered into four dominant patterns in different water type samples. The bacterial genera Pseudomonas, Massilia, Acinetobacter, Sphingomonas, Methylobacterium, and Brevundimonas dominated the finished water, with Brevundimonas and Methylobacterium being speculated to be potential hosts for two ARGs (strA and strB) through network analysis. The enrichment of these two genera, likely caused by selection of disinfection process, may contribute to the higher relative abundance of ARGs in finished water. This study provides insight and effective assessment of the potential risk of ARGs in drinking water treatment processes at the catchment scale.
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页数:12
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共 43 条
[1]   The drinking water treatment process as a potential source of affecting the bacterial antibiotic resistance [J].
Bai, Xiaohui ;
Ma, Xiaolin ;
Xu, Fengming ;
Li, Jing ;
Zhang, Hang ;
Xiao, Xiang .
SCIENCE OF THE TOTAL ENVIRONMENT, 2015, 533 :24-31
[2]   Antibiotics and antibiotic resistance in water environments [J].
Baquero, Fernando ;
Martinez, Jose-Luis ;
Canton, Rafael .
CURRENT OPINION IN BIOTECHNOLOGY, 2008, 19 (03) :260-265
[3]   Presence of antibiotic resistant bacteria and antibiotic resistance genes in raw source water and treated drinking water [J].
Bergeron, Scott ;
Boopathy, Raj ;
Nathaniel, Rajkumar ;
Corbin, Angie ;
LaFleur, Gary .
INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 2015, 102 :370-374
[4]   Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample [J].
Caporaso, J. Gregory ;
Lauber, Christian L. ;
Walters, William A. ;
Berg-Lyons, Donna ;
Lozupone, Catherine A. ;
Turnbaugh, Peter J. ;
Fierer, Noah ;
Knight, Rob .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 :4516-4522
[5]   Contamination of Canadian private drinking water sources with antimicrobial resistant Escherichia coli [J].
Coleman, Brenda L. ;
Louie, Marie ;
Salvadori, Marina I. ;
McEwen, Scott A. ;
Neumann, Norman ;
Sibley, Kristen ;
Irwin, Rebecca J. ;
Jamieson, Frances B. ;
Daignault, Danielle ;
Majury, Anna ;
Braithwaite, Shannon ;
Crago, Bryanne ;
McGeer, Allison J. .
WATER RESEARCH, 2013, 47 (09) :3026-3036
[6]   Network succession reveals the importance of competition in response to emulsified vegetable oil amendment for uranium bioremediation [J].
Deng, Ye ;
Zhang, Ping ;
Qin, Yujia ;
Tu, Qichao ;
Yang, Yunfeng ;
He, Zhili ;
Schadt, Christopher Warren ;
Zhou, Jizhong .
ENVIRONMENTAL MICROBIOLOGY, 2016, 18 (01) :205-218
[7]   Unexpected common occurrence of transferable extended spectrum cephalosporinase-producing Escherichia coil in Swedish surface waters used for drinking water supply [J].
Egervarn, Maria ;
Englund, Stina ;
Ljunge, Marianne ;
Wiberg, Christer ;
Finn, Maria ;
Lindblad, Mats ;
Borjesson, Stefan .
SCIENCE OF THE TOTAL ENVIRONMENT, 2017, 587 :466-472
[8]   Microbiological contamination and resistance genes in biofilms occurring during the drinking water treatment process [J].
Farkas, Anca ;
Butiuc-Keul, Anca ;
Ciataras, Dorin ;
Neamtu, Calin ;
Craciunas, Cornelia ;
Podar, Dorina ;
Dragan-Bularda, Mihail .
SCIENCE OF THE TOTAL ENVIRONMENT, 2013, 443 :932-938
[9]   Detection of Antibiotic Resistance Genes in Source and Drinking Water Samples from a First Nations Community in Canada [J].
Fernando, Dinesh M. ;
Tun, Hein Min ;
Poole, Jenna ;
Patidar, Rakesh ;
Li, Ru ;
Mi, Ruidong ;
Amarawansha, Geethani E. A. ;
Fernando, W. G. Dilantha ;
Khafipour, Ehsan ;
Farenhorst, Annemieke ;
Kumar, Ayush .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2016, 82 (15) :4767-4775
[10]   Isolation and identification of Methylobacterium species from the tap water in hospitals in Japan and their antibiotic susceptibility [J].
Furuhata, K ;
Kato, Y ;
Goto, K ;
Hara, M ;
Yoshida, S ;
Fukuyama, M .
MICROBIOLOGY AND IMMUNOLOGY, 2006, 50 (01) :11-17