Immunologic cross-reactivity between Muscovy duck parvovirus and goose parvovirus on the basis of epitope prediction

被引:7
作者
Li, Ming [1 ]
Yu, Tian-fei [2 ]
机构
[1] Qiqihar Univ, Coll Comp & Control Engn, Qiqihar 161006, Peoples R China
[2] Qiqihar Univ, Coll Life Sci & Agr Forestry, Qiqihar 161006, Peoples R China
关键词
MDPV; GPV; cross-reactivity; prediction; epitope; LINKED-IMMUNOSORBENT-ASSAY; PROTEIN; STRAINS;
D O I
10.1590/S1517-83822013000200031
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Through bioinformatic prediction, between Muscovy duck parvovirus (MDPV) and goose parvovirus (GPV), there were one epitope AA503-509 (RANEPKE) on non-structural protein and three epitopes AA426-430 (SQDLD), 540-544 (DPYRS), 685-691 (KENSKRW) on structural protein might cross-react with each other. Furthermore, the four epitops were expressed in Escherichia coli. All the four recombinant proteins could react with GPV-antisera and MDPV-antisera in Western blot.
引用
收藏
页码:519 / 521
页数:3
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