The Humoral Immune Response to Various Domains of Protective Antigen of Bacillus anthracis in Cutaneous Anthrax Cases in India

被引：4
作者：
Varshney, Anshul [1 ]
Puranik, Nidhi [1 ]
Kumar, M. [1 ]
Goel, A. K. [1 ]
机构：
[1] Def Res & Dev Estab, Div Biotechnol, Gwalior 474002, India
来源：
DEFENCE SCIENCE JOURNAL | 2016年 / 66卷 / 06期
关键词：
Anthrax; Bacillus anthracis; domain; protective antigen; vaccine; edema factor; LINKED-IMMUNOSORBENT-ASSAY; LETHAL FACTOR; TOXIN; HOST; SERODIAGNOSIS; RECEPTOR; BINDING; MODEL;
D O I：
10.14429/dsj.66.10805
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Anthrax, caused by Bacillus anthracis is known to occur globally since antiquity. Besides being an important bio-threat agent, it is an important public health importance pathogen also in countries like India. B. anthracis secretes three distinct toxins, namely protective antigen (PA), lethal factor (LF) and edema factor (EF). PA is the central moiety of the anthrax toxin complex and therefore has been a molecule of choice for vaccine development. PA has four different domains with different functions. In this study, the major domains of PA were cloned and expressed in bacterial system. The purified recombinant proteins were used to determine the humoral immune response by ELISA using 43 human cutaneous anthrax serum samples. The maximum immunoreactivity was observed with the whole PA protein followed by domain 2, 4 and 1. The study corroborated that in addition to full PA, individual domain 2 and 4 can also be good target for vaccine development as well as for serodiagnostic assays for cutaneous anthrax.
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收藏
页码：645 / 650
页数：6
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